Corder C N, Klaniecki T S, McDonald R H, Berlin C
J Pharm Sci. 1975 May;64(5):785-92. doi: 10.1002/jps.2600640511.
A fluorometric method for determining bethanidine in blood plasma is described. The bethanidine is extracted into chloroform, a drug-dye complex with eosin Y is formed, and the fluorescence is measured (excitation, 535 nm; fluorescence, 560 nm). The assay detects 0.02 muM bethanidine (4 ng/ml) in plasma. The relative fluorescence of several body constituents and antihypertensive drugs is negligible. The plasma levels of bethanidine in four hypertensive patients receiving this drug were measured.
本文描述了一种测定血浆中苄乙胍的荧光法。苄乙胍被萃取到氯仿中，与曙红Y形成药物-染料复合物，然后测量荧光（激发波长535nm；发射波长560nm）。该检测方法可检测出血浆中0.02μM的苄乙胍（4ng/ml）。几种人体成分和抗高血压药物的相对荧光可忽略不计。测定了四名接受该药物治疗的高血压患者血浆中苄乙胍的水平。
