巴西卫生部官方采用的血清学检测方法用于诊断接种 Leishmune®疫苗的犬内脏利什曼病的纵向分析。
Longitudinal analysis of serological tests officially adopted by the Brazilian Ministry of Health for the diagnosis of canine visceral leishmaniasis in dogs vaccinated with Leishmune®.
机构信息
Department of Clinics, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, São Paulo 16050-680, Brazil.
出版信息
Vet Parasitol. 2013 Nov 8;197(3-4):649-52. doi: 10.1016/j.vetpar.2013.07.013. Epub 2013 Jul 18.
Development of vaccines against canine visceral leishmaniasis (CVL) may provide a prophylactic barrier, but antibody response detected by standard diagnostic techniques may not separate vaccinated from naturally infected dogs. Moreover, anti-Leishmania antibody levels in vaccinated dogs may be detectable for months. Accordingly, the aim of the present study was to comparatively evaluate an "in-house" ELISA with three serological tests officially adopted by the Brazilian Ministry of Health for the diagnosis of CVL in dogs vaccinated with Leishmune(®). A total of 18 mongrel dogs were submitted to a complete protocol of the vaccine, monitored and evaluated in 5 times (T0-T4) up to 180 days after T0. Twenty-one days after the first dose (T1), 50% of the dogs were seropositive by the "in-house" ELISA and 5.5% by IFAT, while by the official ELISA and DPP(®) CVL rapid test all dogs tested negative. At time T2, 42 days after of the first dose, 100%, 83.3%, 11.1%, and 5.5% of the dogs were seropositive by the "in-house" ELISA, IFAT, official ELISA kit and the DPP(®) CVL rapid test, respectively. Ninety days after the first dose (T3), 100%, 83.3%, 72.2% and 33.3% of the dogs were seropositive by the "in-house" ELISA, official ELISA kit, IFAT, and the DPP(®) CVL rapid test, respectively. Finally, at time T4, 88.8%, 33.3%, 11.1% and 5.5% of the dogs were seropositive by the "in-house" ELISA, official ELISA kit, DPP(®) CVL rapid test and IFAT, respectively. In conclusion, dogs vaccinated with Leishmune(®) cross-react by an "in-house" ELISA and by the three official Brazilian serological tests for the diagnosis of canine visceral leishmaniasis up to six months after the first vaccine dose, and may be mistakenly diagnosed and removed.
犬内脏利什曼病（CVL）疫苗的开发可能提供预防屏障，但标准诊断技术检测到的抗体反应可能无法区分接种疫苗和自然感染的狗。此外，接种疫苗的狗的抗利什曼原虫抗体水平可能在几个月内可检测到。因此，本研究的目的是比较评估一种“内部” ELISA 与巴西卫生部正式采用的三种血清学检测方法，用于诊断用 Leishmune®（利什曼原虫）接种的犬 CVL。总共 18 只杂种狗接受了完整的疫苗方案，在 T0 后 5 次（T0-T4）进行监测和评估，直至 180 天。在第一次剂量后 21 天（T1），50%的狗通过“内部”ELISA 呈血清阳性，5.5%通过 IFAT，而通过官方 ELISA 和 DPP（®）CVL 快速检测，所有狗均为阴性。在 T2 时，即第一次剂量后 42 天，100%、83.3%、11.1%和 5.5%的狗通过“内部”ELISA、IFAT、官方 ELISA 试剂盒和 DPP（®）CVL 快速检测呈血清阳性。第一次剂量后 90 天（T3），100%、83.3%、72.2%和 33.3%的狗通过“内部”ELISA、官方 ELISA 试剂盒、IFAT 和 DPP（®）CVL 快速检测呈血清阳性。最后，在 T4 时，88.8%、33.3%、11.1%和 5.5%的狗通过“内部”ELISA、官方 ELISA 试剂盒、DPP（®）CVL 快速检测和 IFAT 呈血清阳性。总之，用 Leishmune®（利什曼原虫）接种的狗在第一次疫苗接种后六个月内通过“内部”ELISA 和三种巴西官方血清学检测方法发生交叉反应，可能被误诊和去除。
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