Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, DF 11340, Mexico.
Phytomedicine. 2013 Nov 15;20(14):1280-7. doi: 10.1016/j.phymed.2013.07.006. Epub 2013 Aug 3.
In this study, the pharmacological interactions between a Rhodiola rosea ethanol extract and B-vitamins such as thiamine (B1), riboflavine (B2), pyridoxine (B6), cyanocobalamin (B12) and a mixture of vitamins B1+B6+B12 was investigated in the mouse formalin test.
Individual dose response curves of the Rhodiola rosea ethanol extract, as well as B-vitamins alone or in a mixture were evaluated in mice in which nociception was induced with 2% formalin intraplantarly. The antinociceptive mechanisms of the Rhodiola rosea were investigated by exploring the role of the opioid and serotonin receptors and the nitric oxide pathway. Isobolographic analysis was used to evaluate the pharmacological interactions between the Rhodiola rosea ethanol extract and each B-vitamin individually or the mixture of vitamins B1+B6+B12 by using the ED30 and a fixed 1:1 ratio combination.
Administration of the Rhodiola rosea extract alone or in combination with all of the vitamins produced a significant and dose-dependent antinociceptive response. The antinociceptive effect of the Rhodiola rosea extract (ED50=81 mg/kg, p.o.) was significant and reverted in the presence of antagonists of the 5-HT1A, GABA/BDZs and opioid receptors and by blocking mediators of the nitric oxide/cGMP/K(+) channels pathway. Isobolograms demonstrate that all of the combinations investigated in this study produced a synergistic interaction experimental ED30 values were significantly smaller than those calculated theoretically.
These results provide evidence that a Rhodiola rosea ethanol extract in combination with B-vitamins produces a significant diminution in the nociceptive response in a synergistic manner, which is controlled by various mechanisms. These findings could aid in the design of clinical studies and suggest that these combinations could be applied for pain therapy.
本研究旨在探讨红景天乙醇提取物与维生素 B 类（如硫胺素（B1）、核黄素（B2）、吡哆醇（B6）、氰钴胺素（B12））以及维生素 B1+B6+B12 混合物在小鼠福尔马林试验中的药理相互作用。
通过在小鼠足底注射 2%福尔马林诱导痛觉，评估红景天乙醇提取物、维生素 B 类单独或混合使用时的个体剂量反应曲线。通过探索阿片类和 5-羟色胺受体以及一氧化氮途径的作用，研究红景天的镇痛机制。采用等辐射分析法，用 ED30 和固定的 1:1 比例组合，评估红景天乙醇提取物与每种维生素单独或与维生素 B1+B6+B12 混合物的药理相互作用。
单独给予红景天提取物或与所有维生素联合使用均可产生显著的、剂量依赖性的镇痛反应。红景天提取物（ED50=81mg/kg，po）的镇痛作用显著，在 5-HT1A、GABA/BDZs 和阿片受体拮抗剂以及阻断一氧化氮/cGMP/K(+)通道途径的介质存在下，这种作用被逆转。等辐射图谱表明，本研究中研究的所有组合均产生协同相互作用，实验 ED30 值明显小于理论计算值。
这些结果提供了证据，表明红景天乙醇提取物与维生素 B 类联合使用可协同显著减轻疼痛反应，这是由多种机制控制的。这些发现有助于设计临床研究，并表明这些组合可用于疼痛治疗。
