J Biomed Opt. 2013 Aug;18(8):80502. doi: 10.1117/1.JBO.18.8.080502.
A combined two-photon microscopy (TPM) and angiographic optical coherence tomography (OCT) is developed, which can provide molecular, cellular, structural, and vascular information of tissue specimens in vivo. This combined system is implemented by adding an OCT vasculature visualization method to the previous combined TPM and OCT, and then is applied to in vivo tissue imaging. Two animal models, a mouse brain cranial window model and a mouse ear cancer model, are used. Both molecular, cellular information at local regions of tissues, and structural, vascular information at relatively larger regions are visualized in the same sections. In vivo tissue microenvironments are better elucidated by the combined TPM and angiographic OCT.
我们开发了一种双光子显微镜（TPM）和血管造影光学相干断层扫描（OCT）的组合系统，该系统可提供组织标本在体的分子、细胞、结构和血管信息。该组合系统通过在前一个 TPM 和 OCT 的组合系统中添加 OCT 血管可视化方法来实现，然后将其应用于在体组织成像。我们使用了两个动物模型，一个是小鼠脑颅窗模型，另一个是小鼠耳部癌症模型。在同一切片中，可以同时可视化组织局部区域的分子、细胞信息以及较大区域的结构、血管信息。通过组合 TPM 和血管造影 OCT，可以更好地阐明在体组织微环境。
