Department of Oral Biology, Faculty of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Microsc Res Tech. 2013 Nov;76(11):1125-30. doi: 10.1002/jemt.22275. Epub 2013 Aug 7.
The aim of this study is to investigate the histological effect of alcohol ingestion on the regeneration of the submandibular gland (SMG) in rats. Twelve 60-day-old male Wistar rats were randomized into two experimental groups. Test group (TG) animals ingested 40° GL of alcohol for 45 days before surgery, being its concentration gradually increased 10° GL/week for 4 weeks to achieve the final concentration of 40° GL. The control group (CG) received water during the whole experimental period. One-third of the left SMG lobe was removed. Three and seven days after, the whole gland was excised and analyzed. In the TG, the inflammatory process was pronounced when comparing the CG on day 3. The inverse aspect was observed on day 7, associated with an advanced parenchyma development. Changes in laminin expression and glycoproteins production were observed in the TG, causing advanced morphogenesis and delay in cytodifferentiation during the salivary gland regeneration, probably due to alcohol effects. Animals who received ethanol showed alterations in the pattern of glandular regeneration.
本研究旨在探讨酒精摄入对大鼠下颌下腺（SMG）再生的组织学影响。将 12 只 60 日龄雄性 Wistar 大鼠随机分为两组。实验组（TG）动物在手术前 45 天摄入 40°GL 的酒精，其浓度每周增加 10°GL，持续 4 周，达到最终浓度 40°GL。对照组（CG）在整个实验期间接受水。三分之一的左侧 SMG 叶被切除。第 3 天和第 7 天，切除整个腺体进行分析。在 TG 中，与 CG 相比，第 3 天炎症过程明显。第 7 天观察到相反的情况，与更先进的实质发育相关。在 TG 中观察到层粘连蛋白表达和糖蛋白产生的变化，导致唾液腺再生过程中的形态发生提前和细胞分化延迟，这可能是由于酒精的影响。接受乙醇的动物显示腺体再生模式发生改变。
