Fernandes Luanna Melo Pereira, Teixeira Francisco Bruno, Alves-Junior Sergio Melo, Pinheiro João de Jesus Viana, Maia Cristiane Socorro Ferraz, Lima Rafael Rodrigues
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Campus Guamá, Belém, Pará, Brazil.
School of Dentistry, Federal University of Pará, Campus Guamá, Belém, Pará, Brazil.
Histol Histopathol. 2015 Sep;30(9):1069-78. doi: 10.14670/HH-11-604. Epub 2015 Mar 12.
Alcoholism in humans is a chronic and progressive disease, characterized by loss of ethanol consumption control. Previous studies have reported that prolonged exposure to ethanol was responsible for alterations in glandular tissues of human and rodents. However, the interrelationship between ethanol and the glandular system is still the subject of numerous investigations, including the possible resistance of the submandibular gland (SG). In the present study, we investigated whether chronic ethanol exposure during adolescence may affect the parotid gland (PG) and SG in female rats. Female rats (n=16) were treated with distilled water or ethanol (dose of 6.5 g/kg/day, 22.5% w/v) through gavage for 55 days. Glands were collected, weighed and submitted to histological processing. Morphometric analysis was assessed by parenchymal and stromal area measurements. Smooth muscle actin (α-SMA), cytokeratin-19 (CK19) and apoptotic caspase-3 (CAS) were measured using ImageJ® software. Chronic ethanol administration did not alter the body weight of rats after treatment, although it increased glandular weight (p<0.001), reduced the parenchyma area (p<0.001) and decreased CK19 and α-SMA immunostainning in the PG. Besides, ethanol induced CK19 and CAS overexpression, and the occurrence of duct-like structures in SG. These results suggest that ethanol induces histological and morphometric changes in salivary glands of female rats intoxicated with ethanol during adolescence. Furthermore, the mechanism underlying these alterations needs to be investigated but may be not related to the inflammatory process.
人类酒精中毒是一种慢性进行性疾病，其特征是对乙醇摄入失去控制。先前的研究报道，长期接触乙醇会导致人类和啮齿动物腺组织发生改变。然而，乙醇与腺系统之间的相互关系仍是众多研究的主题，包括下颌下腺（SG）可能存在的抗性。在本研究中，我们调查了青春期慢性乙醇暴露是否会影响雌性大鼠的腮腺（PG）和SG。通过灌胃法对16只雌性大鼠用蒸馏水或乙醇（剂量为6.5 g/kg/天，22.5% w/v）处理55天。收集腺体，称重并进行组织学处理。通过实质和间质面积测量进行形态计量分析。使用ImageJ®软件测量平滑肌肌动蛋白（α-SMA）、细胞角蛋白-19（CK19）和凋亡半胱天冬酶-3（CAS）。慢性给予乙醇在处理后并未改变大鼠体重，尽管它增加了腺体重量（p<0.001），减小了实质面积（p<0.001），并降低了PG中CK19和α-SMA的免疫染色。此外，乙醇诱导了CK19和CAS过表达以及SG中出现导管样结构。这些结果表明，乙醇会在青春期乙醇中毒的雌性大鼠唾液腺中诱导组织学和形态计量学变化。此外，这些改变的潜在机制需要进一步研究，但可能与炎症过程无关。
