由不稳定损伤诱导的快速等温 DNA 自我复制。
Rapid, isothermal DNA self-replication induced by a destabilizing lesion.
机构信息
Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2 (Canada) http://www.chem.ualberta.ca/∼gibbsdavis.
出版信息
Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10577-81. doi: 10.1002/anie.201303225. Epub 2013 Aug 6.
PMID:23922255
Abstract
You spin me round: Using a destabilizing abasic site and high concentration of ligase, rapid DNA self-replication in an isothermal ligase chain reaction (LCR) was produced. Both destabilization and rapid ligation are essential for proper LCR replication. This method also provides insight into prebiotic nucleotide replication and is a potential amplification method for biodiagnostics.
摘要
你让我旋转
通过使用不稳定的碱基和高浓度的连接酶，在等温连接酶链反应（LCR）中产生了快速的 DNA 自我复制。碱基不稳定和快速连接对于正确的 LCR 复制都是必不可少的。该方法还提供了对前生物核苷酸复制的深入了解，并且是生物诊断学的一种潜在的扩增方法。
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