基于模板介导连接的光控 DNA 恒温扩增。

Photo-regulatable DNA isothermal amplification by template-mediated ligation.

机构信息

Department of Bio molecular Engineering, Graduate School of Engineering, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Department of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57 Nagatsuta, Midori-ku, Yokohama, Kanagawa 266-8501, Japan.

出版信息

Chem Commun (Camb). 2019 Jan 25;55(8):1080-1083. doi: 10.1039/c8cc09218d. Epub 2019 Jan 8.

DOI:10.1039/c8cc09218d

PMID:30617360

Abstract

By combining azobenzene-tethered oligonucleotides as modulators and poly(l-lysine)-graft-dextran (PLL-g-Dex), a chaperone polymer, to facilitate strand displacement, we successfully developed a photo-regulatable DNA isothermal amplification method. By alternating UV and visible irradiation, linear amplification was achieved. The method enables photo-regulatability and mismatch discrimination in linear amplification of the DNA target.

摘要

通过将偶氮苯键合寡核苷酸作为调节剂和聚（L-赖氨酸）-接枝-葡聚糖（PLL-g-Dex）作为伴侣聚合物结合起来，以促进链置换，我们成功开发了一种光调控的 DNA 等温扩增方法。通过交替进行 UV 和可见光照射，实现了线性扩增。该方法可实现光调控，并在 DNA 靶标的线性扩增中实现错配识别。