Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2013 Jul 29;8(7):e69588. doi: 10.1371/journal.pone.0069588. Print 2013.
A simple method to extract DNA from hair shafts was developed by using enzymatic laundry powder at the first step of the process. The whole extraction can be finished in less than 2 hours. The simple extraction reagent proposed here contains only two cheap components: ordinary enzymatic laundry powder and PCR buffer. After extraction, an ultra sensitive fluorescent nucleic acid stain, PicoGreen, was used for quantifying trace amount of double-stranded DNA in the solution extracted. For further validation of DNA extraction, four primers were employed to amplify DNA microsatellite loci. Both fluorescence spectroscopy and PCR results suggested that this method can extract DNA from hair shafts with good efficiency and repeatability. The study will greatly facilitate the use of hair shafts in future for DNA analyses on genome-wide scale.
我们开发了一种从头发中提取 DNA 的简单方法，该方法在处理过程的第一步使用酶洗衣粉。整个提取过程可以在不到 2 小时内完成。这里提出的简单提取试剂仅包含两种廉价成分：普通的酶洗衣粉和 PCR 缓冲液。提取后，使用超灵敏荧光核酸染色剂 PicoGreen 对提取溶液中的痕量双链 DNA 进行定量。为了进一步验证 DNA 提取，我们使用了四个引物来扩增 DNA 微卫星基因座。荧光光谱和 PCR 结果均表明，该方法可以有效地从头发中提取 DNA，并且具有良好的重复性。该研究将极大地促进未来在全基因组范围内使用头发进行 DNA 分析。
