Hu Yujuan, Kong Weijia, Han Yuechen, Wang Ying, Cheng Huamao, Chen Min
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2004 May;18(5):288-91.
To discuss the method of extracting nucleic acid from rat's hair.
The method with PCR buffer, SDS-proteinase K or Chelex-100 were used to extract the nucleic acid from rat's hair separately, and the isolated nucleic acid was analyzed by PCR and electrophoresis.
The success rate of antracting mt DNA from the hair gollcle, we gound the method with SDS-prot einase kis lower than the ather two methods (chi1(2) = 42.421, chi2(2) = 28.800, P<0.01). This is same as the result ot entracting nuclear DNA (chi1(2) = 49.091, chi2(2) = 30.767, P<0.01). While no alifference has been bound between the method with PCR buffer and with chelex-100 (mtDNA: chi(2) = 0.296; nuclear DNA: chi(2) = 0.048, P>0.05). The method with PCR buffer can't extract nucleic acid from hair shafts, the method with SDS-proteinase K can't extract mtDNA from one or two hairs, the method with Chelex-100 can extract mtDNA and nuclear DNA from single rat's hair or hair shafts.
The method with Chelex-100 is suitable for extracting nucleic acid from hair.
探讨从大鼠毛发中提取核酸的方法。
分别采用含PCR缓冲液、SDS -蛋白酶K或Chelex - 100的方法从大鼠毛发中提取核酸，并通过PCR和电泳对提取的核酸进行分析。
从毛囊中提取线粒体DNA的成功率，我们发现SDS -蛋白酶K法低于其他两种方法（χ1(2)=42.421，χ2(2)=28.800，P<0.01）。提取核DNA的结果也是如此（χ1(2)=49.091，χ2(2)=30.767，P<0.01）。而含PCR缓冲液的方法与含Chelex - 100的方法之间未发现差异（线粒体DNA：χ(2)=0.296；核DNA：χ(2)=0.048，P>0.05）。含PCR缓冲液的方法不能从毛干中提取核酸，含SDS -蛋白酶K的方法不能从一或两根毛发中提取线粒体DNA，含Chelex - 100的方法可从单根大鼠毛发或毛干中提取线粒体DNA和核DNA。
含Chelex - 100的方法适用于从毛发中提取核酸。
