Application of TLC method with video scanning in estimation of daily dietary intake of specific flavonoids--preliminary studies.

Flavonoids, substances present in foods of plant origin, play an important role in many metabolic processes. Numerous properties of these substances were described, including their anti-allergic, antitumor and antioxidant properties. Therefore, an increased intake of these nutrients may play a beneficial role in human health. The aim of the presented study was to estimate the daily intake of specific flavonoid compounds using thin layer chromatography (TLC) combined with densitometric qualitative and quantitative analysis. Performed investigations revealed the presence of two flavonoids in the extracts from daily food rations--naringenin and hesperidin. Naringenin content in the daily food ration of women was calculated to be 179-537 mg, whereas in the group of men it ranged around 181-550 mg, depending on the conducted method of extraction and solvent system used. Daily dietary intake of hesperidin was calculated to be 193-534 mg in the group of women and 194-562 mg in the group of men. The highest degree of extraction of these flavonoids was obtained for the mixture of acetone and water (7 : 3, v/v) by means of accelerated solvent extraction (ASE).