National University of Mar del Plata, Faculty of Engineering, Department of Chemistry, Biochemical Engineering Group, Juan B. Justo 4302, (7600), Mar del Plata, Argentina.
J Environ Sci (China). 2013 Mar 1;25(3):613-25. doi: 10.1016/s1001-0742(12)60020-5.
We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 10(5) colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.
我们报告了一种分离的氯酚降解菌假单胞菌 H 对纯碳氢化合物的生物降解作用和对这些化合物的趋化性。16S rDNA 序列的生化和系统发育分析将假单胞菌 H 鉴定为与铜绿假单胞菌 PA01 具有 99.56%的相似性。该菌株能够降解正十六烷、1-十一烯、1-壬烯、1-癸烯、1-十二烯和煤油。它可以在 1-辛烯存在的情况下生长，而这种碳氢化合物对其他碳氢化合物降解菌是有毒的。假单胞菌 H 对正十六烷、煤油、1-十一烯和 1-十二烯也具有趋化性。这些结果表明，这种假单胞菌 H 是在受控环境中进行含碳氢化合物废水生物修复的有吸引力的候选菌株。由于经典的检测趋化性的标准技术在细菌浓度较低时效率不高，我们证明了动态散斑激光方法的使用，该方法简单且廉价，可在碳氢化合物作为趋化物时确认低细胞浓度（每毫升少于 10^5 个菌落形成单位（CFU/mL））下的细菌趋化性。
