School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
J Environ Sci (China). 2013 Apr 1;25(4):770-5. doi: 10.1016/s1001-0742(12)60046-1.
To accelerate the efficiency of methane biodegradation in landfills, a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, JTA1, which can utilize methane as well as acetate, was isolated from the Laogang MSW landfills, Shanghai, China. Strain JTA1 was a member of genus Methylocystis on the basis of 16S rRNA and pmoA gene sequence similarity. The maximum specific cell growth rates (micro(max) = 0.042 hr(-1), R2 = 0.995) was derived through Boltzmann simulation, and the apparent half-saturation constants (K(m(app)) = 7.08 mmol/L, R2 = 0.982) was calculated according to Michaelis-Menton hyperbolic model, indicating that Methylocystis strain JTA1 had higher-affinity potential for methane oxidation than other reported methanotrophs. By way of adding the strain JTA1 culture, the methane consumption of aged refuse reached 115 mL, almost two times of control experiment. In addition, high tolerance of Methylocystis strain JTA1 to chloroform could facilitate the methane oxidation of aged refuse bio-covers. At the chloroform concentration of 50 mg/L, the methane-oxidation rate of bio-cover reached 0.114 mL/(day x g), much higher than the highest rate, 0.0135 mL/(day x g), of reported bio-covers. In conclusion, strain JTA1 opens up a new possibility for environmental biotechnology, such as soil or landfills bioremediation and wastewater decontamination.
为了提高垃圾填埋场中甲烷生物降解的效率，从中国上海老港垃圾填埋场中分离到一株能够利用甲烷和乙酸盐的革兰氏阴性、杆状、非运动、非孢子形成的细菌 JTA1。根据 16S rRNA 和 pmoA 基因序列相似性，菌株 JTA1 属于甲基球菌属。通过 Boltzmann 模拟得出最大比细胞生长速率（micro(max) = 0.042 hr(-1)，R2 = 0.995），根据 Michaelis-Menton 双曲线模型计算出表观半饱和常数（K(m(app)) = 7.08 mmol/L，R2 = 0.982），表明甲基球菌属 JTA1 菌株对甲烷氧化具有更高的亲和力。通过添加菌株 JTA1 培养物，老化垃圾的甲烷消耗达到 115 mL，几乎是对照实验的两倍。此外，甲基球菌属 JTA1 对三氯甲烷的高耐受性可以促进老化垃圾生物覆盖层中的甲烷氧化。在三氯甲烷浓度为 50 mg/L 时，生物覆盖层的甲烷氧化速率达到 0.114 mL/（天 x g），远高于已报道的生物覆盖层的最高速率 0.0135 mL/（天 x g）。总之，JTA1 株为环境生物技术开辟了新的可能性，例如土壤或垃圾填埋场的生物修复和废水净化。
