Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120-750, Korea.
J Microbiol Biotechnol. 2011 Jul;21(7):753-6. doi: 10.4014/jmb.1102.01055.
A methane-oxidizing bacterium was isolated from the enriched culture of a landfill cover soil. The closest relative of the isolate, designated M6, is Methylocystis sp. Based on a kinetic analysis, the maximum specific methane oxidation rate and saturation constant were 4.93 mmol·g--dry cell weight--1·h⁻¹ and 23 microM, respectively. This was the first time a kinetic analysis was performed using pure methanotrophic culture. The methane oxidation by M6 was investigated in the presence of aromatic (m- and p-xylene and ethylbenzene) or sulfur (hydrogen sulfide, dimethyl sulfide, methanthiol) compounds. The methane oxidation was inhibited by the presence of aromatic or sulfur compounds.
从垃圾填埋覆盖土壤的富集培养物中分离出一株甲烷氧化细菌。分离株命名为 M6，与 Methylocystis sp. 亲缘关系最近。基于动力学分析，最大比甲烷氧化速率和饱和常数分别为 4.93 mmol·g--干细胞重量--1·h⁻¹和 23 microM。这是首次使用纯甲烷营养培养物进行动力学分析。研究了 M6 在存在芳香族（间二甲苯、对二甲苯和乙苯）或硫（硫化氢、二甲基硫、甲硫醇）化合物时的甲烷氧化情况。存在芳香族或硫化合物会抑制甲烷氧化。
