Faculty of Pharmaceutical Sciences, Khon Kaen Univ., Khon Kaen 40002, Thailand; Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Natl. Research Univ.-Khon Kaen Univ., Khon Kaen 40002, Thailand.
J Food Sci. 2013 Sep;78(9):C1363-7. doi: 10.1111/1750-3841.12225. Epub 2013 Aug 7.
Polyclonal antibody against iriflophenone 3-C-β-d-glucoside (IP3G), a major compound from the leaves of Aquilaria spp., was produced for the development of an enzyme-linked immunosorbent assay (ELISA). The results showed that the antibodies were specific for IP3G. The produced antibody has low cross reactivity with iriflophenone 3,5-C-β-d-diglucopyranoside (13%), genkwanin 5-O-β-primeveroside (3.55%) and no cross reactivity found in other compounds. The range of ELISA assay extends from 100 to 1560 ng/mL with coefficient of variation (CV) 1.19% to 2.07% for intra-assay and 3.76% to 7.15% for inter-assay precision levels. The recovery rates of IP3G in the leaves of Aquilaria spp. were in the range of 96.0% to 99.0% with CV 4.50% to 5.32%. A correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of IP3G in the plant samples (R(2) = 0.9321). These results suggest that the developed ELISA method can be applied to determine IP3G content with high specificity, rapidity, and simplicity. The developed immunosorbent assay in this study provides a useful tool for the analysis of IP3G in plant samples and products.
针对瑞福酚酮 3-C-β-D-葡萄糖苷（IP3G）的多克隆抗体，该物质是来自白木香属植物叶子中的主要化合物，被用于开发酶联免疫吸附测定（ELISA）。结果表明，该抗体对 IP3G 具有特异性。该抗体与瑞福酚酮 3,5-C-β-D-二葡萄糖苷（13%）、金合欢素 5-O-β-樱草糖苷（3.55%）的交叉反应性较低，而在其他化合物中未发现交叉反应性。ELISA 测定范围为 100 至 1560ng/mL，日内变异系数（CV）为 1.19%至 2.07%，日间精密度为 3.76%至 7.15%。在白木香属植物叶子中 IP3G 的回收率范围为 96.0%至 99.0%，CV 为 4.50%至 5.32%。当用该方法分析植物样品中的 IP3G 时，ELISA 与高效液相色谱法之间存在相关性（R2=0.9321）。这些结果表明，所开发的 ELISA 方法具有特异性高、快速、简单的特点，可用于测定 IP3G 的含量。本研究中开发的免疫吸附测定法为分析植物样品和产品中的 IP3G 提供了有用的工具。
