具有双丙氨膦抗性基因（bar）和衣霉素抗性基因作为植物启动子分析标记的网关二元载体R4L1pGWB的构建。
Development of gateway binary vectors R4L1pGWB possessing the bialaphos resistance gene (bar) and the tunicamycin resistance gene as markers for promoter analysis in plants.
作者信息
Tanaka Yuji, Shibahara Kenta, Nakagawa Tsuyoshi
机构信息
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University.
出版信息
Biosci Biotechnol Biochem. 2013;77(8):1795-7. doi: 10.1271/bbb.130405. Epub 2013 Aug 7.
We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine:dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA® and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.
我们开发了一系列新的R4L1 Gateway二元载体（R4L1pGWB），其携带双丙氨膦抗性基因（bar）或UDP-N-乙酰葡糖胺：磷酸多萜醇N-乙酰葡糖胺-1-P转移酶（GPT）基因作为选择标记，分别赋予植物对BASTA®和衣霉素的抗性。R4L1pGWB具有attR4-attL1-报告基因，并且可以接受attL4-启动子-attR1入门克隆，以便轻松构建attB4-启动子-attB1-报告基因克隆。新的R4L1pGWB有助于在对卡那霉素或潮霉素具有抗性的现有转基因植物中进行启动子：报告基因分析。
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