带有双丙氨磷抗性基因（bar）作为植物转化选择标记的Gateway二元载体。
Gateway binary vectors with the bialaphos resistance gene, bar, as a selection marker for plant transformation.
作者信息
Nakamura Shinya, Mano Shoji, Tanaka Yuji, Ohnishi Masato, Nakamori Chihiro, Araki Masami, Niwa Tomoko, Nishimura Mikio, Kaminaka Hironori, Nakagawa Tsuyoshi, Sato Yutaka, Ishiguro Sumie
机构信息
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan.
出版信息
Biosci Biotechnol Biochem. 2010;74(6):1315-9. doi: 10.1271/bbb.100184. Epub 2010 Jun 7.
We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.
我们构建了两个系列的Gateway二元载体，即pGWBs和R4pGWBs，它们带有双丙氨膦抗性基因（bar）作为植物转化的选择标记。该系统中使用的报告基因和标签有sGFP、GUS、LUC、EYFP、ECFP、G3GFP、mRFP、TagRFP、6xHis、FLAG、3xHA、4xMyc、10xMyc、GST、T7和TAP。使用在平板上生长和在土壤中生长的幼苗，均成功地利用BASTA筛选出了拟南芥转化体。在含有BASTA或草铵膦铵盐的平板上筛选出了转化的水稻愈伤组织和悬浮培养的烟草细胞。这些载体与现有的携带卡那霉素和潮霉素B抗性的pGWB和R4pGWB载体兼容。
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