Department of Gastroenterology, Xiamen University, Xiamen, China.
Oncol Res. 2013;20(8):377-81. doi: 10.3727/096504013X13657689383012.
Overexpression of Pokemon, which is an erythroid myeloid ontogenic factor protein, occurs in different cancers, including hepatocellular carcinoma (HCC). Pokemon is also reported to have an oncogenic activity in various human cancers. This study investigated the effect of Pokemon knockdown on the regulation of HCC growth. POK shRNA suppressed the expression of Pokemon protein in HepG2 cells compared to the negative control vector-transfected HCC cells. Pokemon knockdown also reduced HCC cell viability and enhanced cisplatin-induced apoptosis in HCC cells. AKT activation and the expression of various cell cycle-related genes were inhibited following Pokemon knockdown. These data demonstrate that Pokemon may play a role in HCC progression, suggesting that inhibition of Pokemon expression using Pokemon shRNA should be further evaluated as a novel target for the control of HCC.
Pokemon 的过表达发生在不同的癌症中，包括肝细胞癌（HCC）。Pokemon 也被报道在各种人类癌症中具有致癌活性。本研究探讨了 Pokemon 敲低对 HCC 生长调控的影响。与阴性对照载体转染的 HCC 细胞相比，POK shRNA 抑制了 HepG2 细胞中 Pokemon 蛋白的表达。Pokemon 敲低还降低了 HCC 细胞的活力，并增强了 HCC 细胞中顺铂诱导的细胞凋亡。Pokemon 敲低后 AKT 激活和各种细胞周期相关基因的表达受到抑制。这些数据表明，Pokemon 可能在 HCC 进展中发挥作用，提示使用 Pokemon shRNA 抑制 Pokemon 表达可能作为 HCC 控制的新靶标进一步评估。
