Yang Yutao, Cui Jiajun, Xue Feng, Overstreet Anne-Marie, Zhan Yiping, Shan Dapeng, Li Hui, Li Hui, Wang Yongjun, Zhang Mengmeng, Yu Chunjiang, Xu Zhi-Qing David
Department of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China.
Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA.
Mol Neurobiol. 2016 Mar;53(2):1266-1278. doi: 10.1007/s12035-014-9081-2. Epub 2015 Jan 27.
POK erythroid myeloid ontogenic factor (Pokemon), an important proto-oncoprotein, is a transcriptional repressor that regulates the expression of many genes and plays an important role in tumorigenesis. Resveratrol (RSV), a natural polyphenolic compound, has many beneficial biological effects on health. In this study, we investigated the role of Pokemon in RSV-induced biological effects and the effect of RSV on the expression of Pokemon in glioma cells. We found that overexpression of Pokemon decreased RSV-induced cell apoptosis, senescence, and anti-proliferative effects. Moreover, we showed that RSV could efficiently decrease the activity of the Pokemon promoter and the expression of Pokemon. Meanwhile, RSV also inhibited Sp1 DNA binding activity to the Pokemon promoter; whereas, it did not influence the expression and nuclear translocation of Sp1. In addition, we found that RSV could increase the recruitment of HDAC1, but decreased p300 to the Pokemon promoter. Taken together, all these results extended our understanding on the anti-cancer mechanism of RSV in glioma cells.
POK红系髓系发育因子（Pokemon）是一种重要的原癌蛋白，是一种转录抑制因子，可调节许多基因的表达，并在肿瘤发生中起重要作用。白藜芦醇（RSV）是一种天然多酚化合物，对健康具有许多有益的生物学作用。在本研究中，我们研究了Pokemon在RSV诱导的生物学效应中的作用以及RSV对胶质瘤细胞中Pokemon表达的影响。我们发现Pokemon的过表达降低了RSV诱导的细胞凋亡、衰老和抗增殖作用。此外，我们表明RSV可以有效降低Pokemon启动子的活性和Pokemon的表达。同时，RSV还抑制Sp1与Pokemon启动子的DNA结合活性；然而，它不影响Sp1的表达和核转位。此外，我们发现RSV可以增加HDAC1与Pokemon启动子的结合，但减少p300与Pokemon启动子的结合。综上所述，所有这些结果扩展了我们对RSV在胶质瘤细胞中的抗癌机制的理解。
