Sect. Behavioural Neuroscience, Istituto Superiore di Sanità, Roma, Italy.
Sci Rep. 2013;3:2380. doi: 10.1038/srep02380.
All laboratory animals shall be provided some form of environmental enrichment (EE) in the nearest future (Directive 2010/63/EU). Displacing standard housing with EE entails the possibility that data obtained under traditional housing may be reconsidered. Specifically, while EE often contrasts the abnormalities of consolidated disease models, it also indirectly demonstrates that their validity depends on housing conditions. We mimicked a situation in which the consequences of a novel pharmacological compound were addressed before and after the adoption of the Directive. We sub-chronically exposed standard- or EE-reared adolescent CD1 mice (postnatal days 23-33) to the synthetic compound JWH-018, and evaluated its short- and long-term potential cannabinoid properties on: weight gain, locomotion, analgesia, motor coordination, body temperature, brain metabolism ((1)H MRI/MRS), anxiety- and depressive-related behaviours. While several parameters are modulated by JWH-018 independently of housing, other effects are environmentally mediated. The transition from standard housing to EE shall be carefully monitored.
所有实验动物在不久的将来都将获得某种形式的环境丰容（EE）（指令 2010/63/EU）。用 EE 取代标准笼具可能会导致重新考虑在传统笼具条件下获得的数据。具体来说，虽然 EE 通常与综合疾病模型的异常情况形成对比，但它也间接表明其有效性取决于饲养条件。我们模拟了一种情况，即在采用指令之前和之后，都对一种新型药理学化合物的后果进行了研究。我们对标准或 EE 饲养的青少年 CD1 小鼠（出生后第 23-33 天）进行亚慢性暴露于合成化合物 JWH-018，并评估其短期和长期潜在的大麻素特性：体重增加、运动、镇痛、运动协调、体温、大脑代谢（1H MRI/MRS）、焦虑和抑郁相关行为。虽然 JWH-018 可以独立于饲养方式调节几个参数，但其他影响则是环境介导的。从标准笼具到 EE 的转变应仔细监测。
