miR-20a 通过靶向早期生长反应因子 2（EGR2）促进胃癌进展。

Involvement of miR-20a in promoting gastric cancer progression by targeting early growth response 2 (EGR2).

机构信息

Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Xinqiao Road, Chongqing 400037, China.

出版信息

Int J Mol Sci. 2013 Aug 6;14(8):16226-39. doi: 10.3390/ijms140816226.

Gastric cancer (GC) is one of the most common cancers, with high incidences in East Asia. microRNAs (miRNAs) play essential roles in the carcinogenesis of GC. miR-20a was elevated in GC, while the potential function of miR-20a was poorly understood. miR-20a expression was examined in GC tissues and cell lines. The effects of miR-20a on the growth, migration, invasion, and chemoresistance of GC cells were examined. Luciferase reporter assay and Western blot were used to screen the target of miR-20a. miR-20a was increased in GC tissues and cell lines. miR-20a promoted the growth, migration and invasion of GC cells, enhanced the chemoresistance of GC cells to cisplatin and docetaxel. Luciferase activity and Western blot confirmed that miR-20a negatively regulated EGR2 expression. Overexpression of EGR2 significantly attenuated the oncogenic effect of miR-20a. miR-20a was involved in the carcinogenesis of GC through modulation of the EGR2 signaling pathway.

胃癌（GC）是最常见的癌症之一，在东亚地区发病率较高。microRNAs（miRNAs）在 GC 的发生发展中起着重要作用。miR-20a 在 GC 中升高，但其潜在功能尚不清楚。本研究检测了 GC 组织和细胞系中 miR-20a 的表达。通过细胞计数试剂盒-8（CCK-8）、平板克隆形成实验、划痕实验和 Transwell 实验检测 miR-20a 对 GC 细胞生长、迁移和侵袭的影响，通过顺铂和多西紫杉醇处理 GC 细胞检测 miR-20a 对 GC 细胞化疗耐药性的影响。荧光素酶报告基因检测和 Western blot 用于筛选 miR-20a 的靶基因。结果显示，miR-20a 在 GC 组织和细胞系中表达上调。miR-20a 促进 GC 细胞的生长、迁移和侵袭，增强 GC 细胞对顺铂和多西紫杉醇的化疗耐药性。荧光素酶活性和 Western blot 证实 miR-20a 负调控 EGR2 的表达。过表达 EGR2 显著减弱了 miR-20a 的致癌作用。miR-20a 通过调节 EGR2 信号通路参与 GC 的发生发展。