Zhu Guilan, Sheng Long, Tong Qunyi
Department of Life Science, Hefei Normal University, Hefei, AnHui, 230061, China,
Bioprocess Biosyst Eng. 2014 Apr;37(4):743-8. doi: 10.1007/s00449-013-1030-3. Epub 2013 Aug 8.
In this study, the effect of H2O2-induced oxidative stress on gellan gum production and cell growth were investigated. Gellan gum production was improved and cell growth was inhibited by H2O2. A multiple H2O2 stresses with different concentrations were developed to optimize gellan gum production. A maximal gellan gum yield (22.52 g/L), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/L H2O2 added at 6, 12, 18, 24 h, respectively. Moreover, UDP-glucose pyrophosphorylase activity and glucosyltransferase activity were increased with H2O2 stresses. This new strategy of multiple H2O2-induced oxidative stresses would be further applied to gellan gum production in future study.
在本研究中，研究了过氧化氢（H2O2）诱导的氧化应激对结冷胶产量和细胞生长的影响。H2O2提高了结冷胶产量并抑制了细胞生长。通过施加不同浓度的多次H2O2应激来优化结冷胶生产。在分别于6、12、18、24小时添加2、2、3、4 mmol/L H2O2的情况下，观察到结冷胶的最大产量（22.52 g/L），比对照高35.58%。此外，UDP-葡萄糖焦磷酸化酶活性和糖基转移酶活性随着H2O2应激而增加。这种多次H2O2诱导氧化应激的新策略将在未来的研究中进一步应用于结冷胶生产。
