Renom G, Bouquety J C, Lanckriet C, Georges A J, Siopathis M R, Martin P M
Institut Pasteur, Bangui.
Res Virol. 1990 May-Jun;141(3):355-63. doi: 10.1016/0923-2516(90)90007-6.
To improve on the diagnosis of human immunodeficiency virus (HIV) infection, 14 children with clinical evidence of AIDS, 86 children less than 15 months old and born to seropositive mothers, and 29 controls were tested for the presence of IgA antibody in tears directed against HIV antigens on Western blots. IgA antibodies in tears against env- and pol-encoded proteins were present in 13 of 14 children with AIDS and in 13 of 86 children born to seropositive mothers. No HIV-specific IgA was observed in tears of the 29 controls. Among the 86 children less than 15 months old, 11 had clinical evidence of AIDS and 7 of them (64%) had HIV-specific IgA in tears. Results show that the demonstration of lacrimal HIV-specific IgA may help to distinguish between truly infected children and those whose HIV antibodies are passively transferred from the mother. Therefore, this simple method is a good tool for laboratory diagnosis of paediatric AIDS.
为了改进人类免疫缺陷病毒（HIV）感染的诊断方法，对14名有艾滋病临床证据的儿童、86名15个月以下且母亲为血清阳性的儿童以及29名对照者进行检测，以确定泪液中针对Western印迹上HIV抗原的IgA抗体是否存在。14名艾滋病儿童中有13名以及86名母亲为血清阳性的儿童中有13名泪液中存在针对env和pol编码蛋白的IgA抗体。29名对照者的泪液中未观察到HIV特异性IgA。在86名15个月以下的儿童中，11名有艾滋病临床证据，其中7名（64%）泪液中有HIV特异性IgA。结果表明，泪液中HIV特异性IgA的检测可能有助于区分真正感染的儿童和那些HIV抗体由母亲被动转移而来的儿童。因此，这种简单方法是儿科艾滋病实验室诊断的良好工具。
