Renom G, Bouquety J C, Lanckriet C, Georges A J, Siopathis M R, Martin P M
Institut Pasteur, Bangui.
Res Virol. 1990 Sep-Oct;141(5):557-62. doi: 10.1016/0923-2516(90)90086-x.
In order to improve the diagnosis of HIV infection in children born to seropositive mothers, 86 children were previously tested by Western blotting for anti-HIV IgA in tears and IgG in serum, at a median age of 9.2 months. To determine the exact value of the assay, 68/86 children of the same cohort were retested 9 months later. Nine children (13.4%) were seropositive and all had anti-HIV IgA in tears. Eight of them had possessed lachrymal antibodies 9 months earlier. The ninth child was seronegative when 9 months old and then seroconverted. Four children (6%), known to be seronegative, had an indeterminate Western blot pattern and no HIV IgA in tears. Fifty four (80.6%) were seronegative at 18 months; none of them had ever had anti-HIV IgA in tears. This highlights the fact that only the children without lachrymal HIV IgA at the age of 9 months became seronegative at the age of 18 months. Our results clearly show that the detection of anti-HIV IgA in tears is a highly specific and reliable diagnostic test in children aged less than 15 months, born to seropositive mothers.
为了改善对血清反应阳性母亲所生儿童的HIV感染诊断，此前对86名儿童进行了检测，通过蛋白质印迹法检测其泪液中的抗HIV IgA和血清中的IgG，这些儿童的中位年龄为9.2个月。为了确定该检测方法的准确价值，对同一队列中的68/86名儿童在9个月后进行了重新检测。9名儿童（13.4%）血清反应阳性，且所有儿童泪液中均有抗HIV IgA。其中8名儿童在9个月前就已拥有泪液抗体。第9名儿童9个月大时血清反应阴性，之后发生了血清转化。4名已知血清反应阴性的儿童蛋白质印迹结果不确定，且泪液中无HIV IgA。54名儿童（80.6%）在18个月时血清反应阴性；他们中没有人泪液中曾有过抗HIV IgA。这突出了一个事实，即只有9个月大时泪液中没有HIV IgA的儿童在18个月时才会血清反应阴性。我们的结果清楚地表明，检测泪液中的抗HIV IgA对血清反应阳性母亲所生的15个月以下儿童来说是一种高度特异且可靠的诊断检测方法。
