Higuchi Masato, Miura Chiemi, Iwai Toshiharu, Miura Takeshi
South-Ehime Fisheries Research Center, Ehime University, Ainan, Ehime, Japan.
Biol Reprod. 2013 Sep 19;89(3):58. doi: 10.1095/biolreprod.113.109777. Print 2013 Sep.
Meiosis is a unique and critical process in reproduction. Although the key molecular components of meiosis have been identified, the molecular mechanisms regulating the entry into this pathway remain unclear. We previously demonstrated that a progestin in teleost fish, 17alpha, 20beta-dihydroxy-4-pregnen-3-one, is essential for meiotic initiation, and up-regulates taurine synthesis and the production of trypsin in Sertoli cells. In the present study, we found that trypsin promotes the uptake of taurine into germ cells through the up-regulation of solute carrier family 6 (neurotransmitter transporter, taurine), member 6 (Slc6a6) expression. We further found that this up-regulation of the taurine signal is required for Spo11a expression and meiotic initiation.
减数分裂是生殖过程中一个独特且关键的过程。尽管减数分裂的关键分子成分已被确定，但调控进入该途径的分子机制仍不清楚。我们之前证明，硬骨鱼中的一种孕激素，17α, 20β - 二羟基 - 4 - 孕烯 - 3 - 酮，对减数分裂起始至关重要，并上调支持细胞中牛磺酸的合成和胰蛋白酶的产生。在本研究中，我们发现胰蛋白酶通过上调溶质载体家族6（神经递质转运体，牛磺酸）成员6（Slc6a6）的表达促进牛磺酸进入生殖细胞。我们进一步发现，牛磺酸信号的这种上调对于Spo11a表达和减数分裂起始是必需的。
