Dorostghoal Mehran, Mahabadi Mahmood Khaksari, Adham Sahar
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahwaz, Ahwaz, Iran.
J Reprod Infertil. 2011 Jan;12(1):15-22.
In recent years concerns have been raised about human reproductive disorders, specially the effects of environmental factors on human fertility and pregnancy outcome. Therefore, the present study was designed to assess the effects of maternal caffeine consumption on ovarian follicles development in rat offspring.
60 pregnant female rats were randomly divided into a control and two experimental groups. The rats in the two experimental groups received caffeine via drinking water during gestation (26 and 45 mg/kg) and lactation (25 and 35 mg/kg). The ovaries of the offspring were removed at 7, 14, 28, 60, 90 and 120 days after birth, and fixed in Bouin's solution. By preparing serial tissue sections, structural changes in ovarian follicles and corpora lutea were studied during postnatal development.
The weight of ovaries decreased significantly (p<0.05) in the high dose caffeine-treated group at all stages of postnatal development. Significant (p<0.05) decreases were seen in the number of primordial follicles from day 7 to 120 after birth in the high dose caffeine-treated group. Moreover, the number of primary and secondary follicles decreased significantly on days 7, 14 and 28 as did the number of antral follicles on days 14 and 28 after birth (p<0.05) in the high dose caffeine-treated group. The diameter of secondary and antral follicles decreased significantly (p<0.05) in high dose caffeine-treated group on the early days of postnatal development. No statistically significant differences were seen in the number of corpora lutea between the groups.
The present study shows that caffeine consumption during gestation and lactation affects the early stages of ovarian follicle development and reduces reproductive efficiency in the offspring of Wistar rats.
近年来，人们对人类生殖紊乱问题日益关注，特别是环境因素对人类生育能力和妊娠结局的影响。因此，本研究旨在评估母体摄入咖啡因对大鼠后代卵巢卵泡发育的影响。
将60只怀孕的雌性大鼠随机分为对照组和两个实验组。两个实验组的大鼠在妊娠期（26和45毫克/千克）和哺乳期（25和35毫克/千克）通过饮水摄入咖啡因。在后代出生后7、14、28、60、90和120天摘除其卵巢，并固定于波因氏液中。通过制备连续组织切片，研究产后发育过程中卵巢卵泡和黄体的结构变化。
高剂量咖啡因处理组在产后发育的各个阶段卵巢重量均显著降低（p<0.05）。高剂量咖啡因处理组出生后7天至120天原始卵泡数量显著减少（p<0.05）。此外，高剂量咖啡因处理组在出生后7天、14天和28天初级和次级卵泡数量显著减少，出生后14天和28天窦状卵泡数量也显著减少（p<0.05）。高剂量咖啡因处理组在产后发育早期次级和窦状卵泡直径显著减小（p<0.05）。各组之间黄体数量未见统计学显著差异。
本研究表明，妊娠期和哺乳期摄入咖啡因会影响卵巢卵泡发育的早期阶段，并降低Wistar大鼠后代的生殖效率。
