Ozbilgin Kemal, Boz Banu, Tuğyan Kazım, Inan Sevinç, Vatansever Seda
Department of Histology and Embryology, School of Medicine, Celal Bayar University, Manisa, Turkey.
J Reprod Infertil. 2012 Jul;13(3):131-7.
Receptor for hyaluronic acid mediated motility (RHAMM) has intracellular and extracellular functions. In this study, we focus on the expression of RHAMM in the rat uterus during estrous cycle and implantation period.
The female adult rats were divided into six groups following estrous cycle determination (n = 36). The utreri of rats were collected according to estrous cycle phases (menstruation group). For the implantation groups, uteri were obtained on D4, D5 and D6 (day of implantation) of pregnancy. The tissue samples were fixed and cut into 5 µm thick sections. RHAMM was investigated using immunohisto-chemical techniques and the intensity of RHAMM was evaluated by using the H-score technique. Comparisons between groups were performed using Kruskal-Wallis test.
The RHAMM immunoreactivity of uterine antimesometrial epithelium (343.00±12.81), mesometrial subepithelium (285.00±27.26) and mesometrial stroma (270.00±36.00) were more prominent (p < 0.05) in the proestrus than estrus (275.00± 25.96; 220.00±14.48; 218.00±11.19) and diestrus (262.00±20.71; 192.50± 29.25; 216.00±12.97) groups, respectively. The most intense staining was seen in the epithelium on day four (275.50±30.06) and six (293.50±34.47) of pregnancy (p < 0.05). Strong RHAMM expressions were in both mature and predecidual cells on D5 (256.00±18.71), (247.50±22.14) and D6 (256.00±30.72), (265.00±14.87), respectively. RHAMM expression was prominent in the nondecidual region on D5 (270.00± 13.36).
Considering the role of RHAMM in cell proliferation, differentiation and angiogenesis, spatiotemporal expression of RHAMM in the uterus during estrous cycle and peri-implantation period is a means through which uterus becomes receptive for developing an embryo.
透明质酸介导的运动受体（RHAMM）具有细胞内和细胞外功能。在本研究中，我们聚焦于发情周期和植入期大鼠子宫中RHAMM的表达。
成年雌性大鼠在确定发情周期后分为六组（n = 36）。根据发情周期阶段收集大鼠子宫（月经期组）。对于植入组，在妊娠第4、5和6天（植入日）获取子宫。组织样本固定后切成5μm厚的切片。采用免疫组织化学技术检测RHAMM，并使用H评分技术评估RHAMM的强度。组间比较采用Kruskal-Wallis检验。
发情前期子宫反系膜上皮（343.00±12.81）、系膜上皮下（285.00±27.26）和系膜基质（270.00±36.00）的RHAMM免疫反应性比发情期（275.00±25.96；220.00±14.48；218.00±11.19）和动情后期（262.00±20.71；192.50±29.25；216.00±12.97）组更显著（p < 0.05）。妊娠第4天（275.50±30.06）和第6天（293.50±34.47）上皮中染色最强（p < 0.05）。在第5天（256.00±18.71）、（247.50±22.14）以及第6天（256.00±30.72）、（265.00±14.87）的成熟细胞和前蜕膜细胞中均有较强的RHAMM表达。第5天在非蜕膜区域RHAMM表达显著（270.00±13.36）。
考虑到RHAMM在细胞增殖、分化和血管生成中的作用，发情周期和植入期周围子宫中RHAMM的时空表达是子宫对胚胎发育变得具有接受性的一种方式。
