Tavukcuoglu Safak, Al-Azawi Tahani, Al-Hasani Safaa, Khaki Amir Afshin, Khaki Arash, Tasdemir Seval
Reproductive Medicine Unit, University of Schleswig-Holstein, Luebeck, Germany.
J Reprod Infertil. 2013 Apr;14(2):79-84.
We performed this study to evaluate use of fresh and frozen sperm samples in non-obstructive azoospermia microdissection testicular sperm extraction (micro-TESE-ICSI) treatment.
We performed a total of 82 consecutive in vitro fertilization (IVF) cycles at Fertijin IVF Center in Istanbul, Turkey from January 2010 to March 2012. In 43 participants we used fresh sperm and frozen sperm in the remaining 39 cases. We used fresh and frozen thawed micro surgical testicular sperm extraction (micro TESE) sperm for ICSI with metaphase II (MII) oocytes.
Frozen microTESE sperm was used in 39 cycles, while 43 ICSI cycles were performed using fresh microTESE. Neither the age of male partners (38.33±5.93 and 38.13±8.28) nor that of the female participants (33.16±6.38 and 33.33±6.97) showed significant difference between fresh versus the microTESE and frozen treatment groups, respectively. FSH concentrations were (14.66±13.93 mIU/ml) in fresh TESE group and (17.91±16.29 mIU/ml) in frozen group with no correlations or differences between the two groups. The average number of mature oocytes injected with sperm was 9.23±3.77, versus 9.26±5.26 in cycles using fresh and frozen microTESE sperm, respectively. Fertilization rate was not significantly different in the fresh microTESE (44.79%) than frozen TESE sperm group (46.76%). The average number of transferred embryos was 1.60±0.49 in fresh sperm group and 1.59±0.50 in frozen sperm group. All embryo transfers were performed on day 3.
Cryopreservation of testicular sperm tissues is more suitable and of great benefite if carried out before ovulation induction and not after, especially in cases with non-obstructive azoospermia.
我们开展这项研究以评估新鲜和冷冻精子样本在非梗阻性无精子症显微取精术（micro-TESE-ICSI）治疗中的应用。
2010年1月至2012年3月期间，我们在土耳其伊斯坦布尔的Fertijin体外受精中心连续进行了82个体外受精（IVF）周期。43名参与者使用新鲜精子，其余39例使用冷冻精子。我们将新鲜和冷冻解冻后的显微外科睾丸精子提取（micro TESE）精子用于与中期II（MII）卵母细胞的卵胞浆内单精子注射（ICSI）。
39个周期使用冷冻microTESE精子，43个ICSI周期使用新鲜microTESE精子。新鲜microTESE组与冷冻治疗组男性伴侣年龄（分别为38.33±5.93和38.13±8.28）及女性参与者年龄（分别为33.16±6.38和33.33±6.97）均无显著差异。新鲜TESE组促卵泡生成素（FSH）浓度为（14.66±13.93 mIU/ml），冷冻组为（17.91±16.29 mIU/ml），两组间无相关性或差异。注射精子的成熟卵母细胞平均数量分别为9.23±3.77和9.26±5.26，新鲜microTESE精子组与冷冻microTESE精子组的受精率无显著差异（分别为44.79%和46.76%）。新鲜精子组移植胚胎平均数量为1.60±0.49，冷冻精子组为1.59±0.50。所有胚胎移植均在第3天进行。
睾丸精子组织冷冻保存更适合且益处显著，若在促排卵前而非促排卵后进行，尤其对于非梗阻性无精子症患者。
