Dafopoulos Konstantinos, Griesinger Georg, Schultze-Mosgau Askan, Orief Yasser, Schöpper Beate, Nikolettos Nikos, Diedrich Klaus, Al-Hasani Safaa
Department of Obstetrics and Gynaecology, University of Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.
Reprod Biomed Online. 2005 Apr;10(4):461-6. doi: 10.1016/s1472-6483(10)60821-8.
The aim of the present study was to describe a simplified and inexpensive method of testicular tissue freezing, to assess the cumulative clinical pregnancy rate (CPR) by this technique, and to provide useful information for counselling couples with non-obstructive azoospermia. One hundred and sixty-five couples with non-obstructive azoospermic males pursuing assisted conception, from December 1995 to December 2002, were included. In all cases, the testicular tissue retrieved by open multiple-biopsy (both sides, by testicular sperm extraction) was frozen using a simple liquid nitrogen vapour freezing technique and was stored in liquid nitrogen thereafter. Only mature spermatozoa were used for intracytoplasmic sperm injection (ICSI) after thawing. Expected CPR were calculated using the Kaplan-Meier survival analysis. A total of 281 cycles were performed resulting in 53 clinical pregnancies. Crude and expected CPR (95% confidence intervals) after three cycles were 32.1 (25.7-40.1) and 55.7% (37.0-74.4) respectively. In conclusion, this simplified method for freezing testicular tissue resulted in a satisfactory outcome after ICSI in cases of non-obstructive azoospermia.
本研究的目的是描述一种简化且经济的睾丸组织冷冻方法，评估该技术的累积临床妊娠率（CPR），并为咨询非梗阻性无精子症夫妇提供有用信息。纳入了1995年12月至2002年12月期间165对有非梗阻性无精子症男性且寻求辅助受孕的夫妇。在所有病例中，通过开放式多点活检（双侧，经睾丸精子提取）获取的睾丸组织采用简单的液氮蒸汽冷冻技术进行冷冻，随后储存在液氮中。解冻后仅使用成熟精子进行卵胞浆内单精子注射（ICSI）。使用Kaplan-Meier生存分析计算预期CPR。共进行了281个周期，产生了53例临床妊娠。三个周期后的粗CPR和预期CPR（95%置信区间）分别为32.1（25.7 - 40.1）和55.7%（37.0 - 74.4）。总之，这种冷冻睾丸组织的简化方法在非梗阻性无精子症病例的ICSI后产生了令人满意的结果。
