Barua Chandana C, Begum Shameem A, Barua Acheenta G, Borah Rumi S, Lahkar Mangala
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022,
Indian J Exp Biol. 2013 Jun;51(6):450-7.
Anxiety related disorders are the most common mental illnesses and major cause of disability in man. Anxiolytic activity of methanol extract of leaves of A. brasiliana (L.) Kuntze (MEAB) was evaluated using hole board (HB), open field (OF), elevated plus maze (EPM) and light/dark exploration test (LDE) in mice. Its locomotor activity was studied using actophotometer and anticonvulsant effect was studied using maximal electroshock-induced seizures and pentylenetetrazole-induced seizures in mice. Single oral administration of MEAB at different doses (100, 300 and 600 mg/kg, ip) significantly increased the number and duration of head poking in the HB test; rearing, assisted rearing and number of square traveled in the OF test; entries and time spent in open arm in the EPM test; time spent in lighted box, and numbers of crossings and transfer latency time in the LDE test. There was significant reduction in the time spent in close arm in the EPM test and time spent in dark box in LDE test. In the actophotometer, the activity count was reduced in MEAB and diazepam treated group than control group. All the three doses of the extract significantly reduced the duration of seizures induced by pentylenetetrazole (chemoshock convulsion). However, the extract did not show any appreciable effect in electroshock convulsion model. The results of the present study suggest promising anxiolytic and anticonvulsant activity of MEAB which might be accredited to different phytoconstituents like alkaloids, steroids and triterpenes present in the methanol extract of A. brasiliana.
焦虑相关障碍是人类最常见的精神疾病和致残的主要原因。使用小鼠的洞板试验（HB）、旷场试验（OF）、高架十字迷宫试验（EPM）和明暗箱试验（LDE）评估了巴西含羞草（A. brasiliana (L.) Kuntze）叶甲醇提取物（MEAB）的抗焦虑活性。使用光电计研究其运动活性，并使用小鼠最大电休克诱导惊厥和戊四氮诱导惊厥研究其抗惊厥作用。不同剂量（100、300和600 mg/kg，腹腔注射）单次口服MEAB可显著增加洞板试验中头部探洞的次数和持续时间；旷场试验中的竖毛、辅助竖毛和走过的方格数；高架十字迷宫试验中进入开放臂的次数和在开放臂停留的时间；明暗箱试验中在亮箱停留的时间、穿越次数和转移潜伏期。高架十字迷宫试验中在封闭臂停留的时间和明暗箱试验中在暗箱停留的时间显著减少。在光电计试验中，MEAB和地西泮治疗组的活动计数低于对照组。提取物的所有三个剂量均显著缩短了戊四氮诱导惊厥（化学休克惊厥）的持续时间。然而，提取物在电休克惊厥模型中未显示出任何明显效果。本研究结果表明MEAB具有良好的抗焦虑和抗惊厥活性，这可能归因于巴西含羞草甲醇提取物中存在的生物碱、甾体和三萜等不同植物成分。
