USDA, Agricultural Research Service, Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX 780280-09184, USA.
J Med Entomol. 2013 Jul;50(4):925-30. doi: 10.1603/me12274.
The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs (miRNAs) are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies. The current study reports discovery ad initial comparative analysis of 88 presumptive miRNA sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nt in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.
厩螫蝇（Stomoxys calcitrans（L.））是一种严重的外寄生虫，会影响动物的生产和动物及人类的健康。厩螫蝇的防治主要依赖于化学杀虫剂；然而，杀虫剂的抗药性发展以及环境方面的考虑因素需要不断的发现研究，以开发新的控制技术。微 RNA（miRNA）是一类短的非编码 RNA，已被证明在广泛的生物体中是基因表达的重要调控因子，并且可能为开发更安全、更有针对性的控制技术提供一种创新的方法。本研究报告了使用高通量测序小 RNA 从厩螫蝇中获得的 88 个假定 miRNA 序列的发现和初步比较分析。厩螫蝇的大多数 miRNA 长度为 22-23nt。许多 miRNA 是节肢动物特有的，一些成熟的 miRNA 序列与蚊子等其他吸血双翅目昆虫的 miRNA 具有更高的序列同一性，而不是与果蝇属的 miRNA。对稳定蝇 miRNAome 进行特征描述的这一初步步骤为进一步分析特定生活阶段和组织特异性表达提供了基础，以阐明它们在稳定蝇生物学中的功能作用。
