Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Phytopathology. 2013 Sep;103(9):960-5. doi: 10.1094/PHYTO-08-12-0188-R.
Cucurbit chlorotic yellows virus (CCYV) (family Closteroviridae, genus Crinivirus) is an emerging virus which causes severe diseases on melon (Cucumis melo) plants. CCYV-infected melon plants display yellowing, mottling, chlorosis, or chlorotic spots on leaves. To develop a new control strategy, the potential for 1,2,3-benzothiadiazole-7-thiocarboxylic acid-S-methyl-ester (ASM) to suppress CCYV infection was evaluated. ASM treatment on melon plants greatly increased the expression levels of pathogenesis-related 1a gene, a marker gene for systemic acquired resistance. ASM treatment on melon plants before inoculation of CCYV suppressed systemic symptoms and decreased CCYV accumulation. ASM treatment on melon even after inoculation of CCYV reduced disease severity and accumulation levels of CCYV. The results show the potential for ASM treatment on attenuation of the CCYV disease symptoms.
葫芦科杂色病毒（CCYV）（家族：芜菁黄花叶病毒科，属：菜豆黄花叶病毒属）是一种新兴病毒，可导致甜瓜（Cucumis melo）植物严重发病。CCYV 感染的甜瓜植株叶片出现黄化、斑驳、褪绿或出现褪绿斑。为开发新的防治策略，评估了 1,2,3-苯并噻二唑-7-硫代羧酸-S-甲酯（ASM）抑制 CCYV 感染的潜力。ASM 处理甜瓜植株可显著提高病程相关 1a 基因的表达水平，该基因为系统获得抗性的标记基因。在接种 CCYV 之前对甜瓜植株进行 ASM 处理可抑制系统症状并减少 CCYV 积累。即使在接种 CCYV 后对甜瓜植株进行 ASM 处理也可降低病害严重度和 CCYV 积累水平。结果表明，ASM 处理可能减轻 CCYV 病症状。
