Department of Genetics, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland.
Plant Biol (Stuttg). 2014 Mar;16(2):476-84. doi: 10.1111/plb.12066. Epub 2013 Aug 8.
It is well known that the process of plant cell differentiation depends on the symplasmic isolation of cells. Before starting the differentiation programme, the individual cell or group of cells should restrict symplasmic communication with neighbouring cells. We tested the symplasmic communication between epidermal cells in the different root zones of parental barley plants Hordeum vulgare L., cv. 'Karat' with normal root hair development, and two root hairless mutants (rhl1.a and rhl1.b). The results clearly show that symplasmic communication was limited during root hair differentiation in the parental variety, whereas in both root hairless mutants epidermal cells were still symplasmically connected in the corresponding root zone. This paper is the first report on the role of symplasmic isolation in barley root cell differentiation, and additionally shows that a disturbance in the restriction of symplasmic communication is present in root hairless mutants.
众所周知，植物细胞分化的过程取决于细胞的共质体隔离。在开始分化程序之前，单个细胞或细胞群应该限制与相邻细胞的共质体通讯。我们测试了父本大麦（Hordeum vulgare L.， cv. 'Karat'）植株不同根区表皮细胞之间的共质体通讯，这些大麦植株具有正常的根毛发育，以及两个根毛缺失突变体（rhl1.a 和 rhl1.b）。结果清楚地表明，在父本品种的根毛分化过程中，共质体通讯受到限制，而在两个根毛缺失突变体中，相应根区的表皮细胞仍然通过共质体相连。本文首次报道了共质体隔离在大麦根细胞分化中的作用，并进一步表明，在根毛缺失突变体中存在共质体通讯限制的干扰。
