Uhaa I J, Riemann H P, Thurmond M C, Franti C E
Department of Epidemiology and Preventive Medicine, University of California, Davis 95616.
Vet Res Commun. 1990;14(4):279-85. doi: 10.1007/BF00350710.
Between December 1985 and March 1987 an enzyme-linked immunosorbent assay (ELISA) was used with 3774 sera to estimate the prevalence of antibodies to Mycoplasma bovis in sera from three age groups of cattle in four dairies in California and to test for possible associations between the presence of M. bovis antibodies and the age or breed of the cattle and the farm. Unadjusted and adjusted associations were evaluated using the chi-square test for associations and multiple logistic regression analysis, respectively. There was a tendency for the proportion of cattle seropositive for M. bovis to increase steadily and approximately linearly with age (p less than 0.05). There was also a statistically significant relationship between a M. bovis seropositive test and being from Farm IV (p less than 0.05). Farm IV was the largest of the four dairies and this association may be due to the effect of herd size. These findings confirm the ubiquitous distribution of antibodies to M. bovis in dairy cattle in California and also support previous reports of herd size as an important factor in mycoplasmal mastitis.
1985年12月至1987年3月期间，采用酶联免疫吸附测定法（ELISA）对加利福尼亚州四个奶牛场三个年龄组的3774份牛血清进行检测，以评估牛血清中抗牛支原体抗体的流行情况，并检测牛支原体抗体的存在与牛的年龄、品种及养殖场之间可能存在的关联。分别使用卡方检验和多元逻辑回归分析来评估未调整和调整后的关联。牛血清中牛支原体呈血清阳性的比例有随年龄稳步且近似线性增加的趋势（p<0.05）。牛支原体血清阳性检测结果与来自IV号养殖场之间也存在统计学上的显著关系（p<0.05）。IV号养殖场是四个奶牛场中最大的，这种关联可能是由于畜群规模的影响。这些发现证实了加利福尼亚州奶牛中抗牛支原体抗体的普遍分布，也支持了之前关于畜群规模是支原体性乳腺炎重要因素的报道。
