National Veterinary Institute (SVA), Uppsala, Sweden.
Wageningen Bioveterinary Research, P.O. Box 65, 8200, AB, Lelystad, the Netherlands.
BMC Vet Res. 2019 Oct 25;15(1):369. doi: 10.1186/s12917-019-2117-0.
Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (Se) and specificity (Sp) of two commercially available ELISA tests (ID Screen® ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of M. bovis infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer's instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of Se and Sp values using latent class analysis.
The Se of WB, the ID Screen® ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding Sp of the three tests were 99.6, 98.6 and 89.6%, respectively.
The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen® ELISA. Based on our results, the ID Screen® ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.
牛支原体(M. bovis)是一种新兴的牛病原体,在全球范围内给畜牧业带来了重大的经济损失。感染可导致多种临床症状,如关节炎、肺炎、乳腺炎和角膜结膜炎,但这些症状都不是 M. bovis 特有的。因此,实验室诊断很重要。检测 M. bovis 抗体的血清学检测被认为是畜群感染的有效指标,常用于畜群检测。结合临床判断,它还可用于实施控制策略和/或估计一个国家的疾病流行率。然而,由于缺乏对检测方法的协调,以及不同实验室使用的血清学检测方法不同,因此,国家间的流行率数据往往难以比较。来自六个欧洲国家的研究人员网络设计并参与了一项实验室间试验,旨在评估两种市售 ELISA 检测(ID Screen® ELISA(IDvet)和 BIO K302 ELISA(BIO-X Diagnostics))诊断 M. bovis 感染的敏感性(Se)和特异性(Sp)。每个实验室都收到了一组盲法牛血清,并按照制造商的说明独立进行了检测。其中一个参与实验室进行的 Western blot 分析(WB)被用作统计评估 Se 和 Sp 值的第三个诊断测试,使用潜在类别分析。
WB、ID Screen® ELISA 和 BIO K302 ELISA 的 Se 分别为 91.8%、93.5%和 49.1%,相应的 Sp 分别为 99.6%、98.6%和 89.6%。
据我们所知,本研究是首次对 BIO K302 ELISA 和 ID Screen® ELISA 进行实验室间比较。根据我们的结果,ID Screen® ELISA 与 WB 具有高度一致性,并且比 BIO K302 ELISA 具有更高的精度和准确性。