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一项采用潜在类别分析评估三种血清学方法用于牛支原体感染诊断的欧洲实验室间试验。

A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis.

机构信息

National Veterinary Institute (SVA), Uppsala, Sweden.

Wageningen Bioveterinary Research, P.O. Box 65, 8200, AB, Lelystad, the Netherlands.

出版信息

BMC Vet Res. 2019 Oct 25;15(1):369. doi: 10.1186/s12917-019-2117-0.

Abstract

BACKGROUND

Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (Se) and specificity (Sp) of two commercially available ELISA tests (ID Screen® ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of M. bovis infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer's instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of Se and Sp values using latent class analysis.

RESULTS

The Se of WB, the ID Screen® ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding Sp of the three tests were 99.6, 98.6 and 89.6%, respectively.

CONCLUSIONS

The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen® ELISA. Based on our results, the ID Screen® ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.

摘要

背景

牛支原体(M. bovis)是一种新兴的牛病原体,在全球范围内给畜牧业带来了重大的经济损失。感染可导致多种临床症状,如关节炎、肺炎、乳腺炎和角膜结膜炎,但这些症状都不是 M. bovis 特有的。因此,实验室诊断很重要。检测 M. bovis 抗体的血清学检测被认为是畜群感染的有效指标,常用于畜群检测。结合临床判断,它还可用于实施控制策略和/或估计一个国家的疾病流行率。然而,由于缺乏对检测方法的协调,以及不同实验室使用的血清学检测方法不同,因此,国家间的流行率数据往往难以比较。来自六个欧洲国家的研究人员网络设计并参与了一项实验室间试验,旨在评估两种市售 ELISA 检测(ID Screen® ELISA(IDvet)和 BIO K302 ELISA(BIO-X Diagnostics))诊断 M. bovis 感染的敏感性(Se)和特异性(Sp)。每个实验室都收到了一组盲法牛血清,并按照制造商的说明独立进行了检测。其中一个参与实验室进行的 Western blot 分析(WB)被用作统计评估 Se 和 Sp 值的第三个诊断测试,使用潜在类别分析。

结果

WB、ID Screen® ELISA 和 BIO K302 ELISA 的 Se 分别为 91.8%、93.5%和 49.1%,相应的 Sp 分别为 99.6%、98.6%和 89.6%。

结论

据我们所知,本研究是首次对 BIO K302 ELISA 和 ID Screen® ELISA 进行实验室间比较。根据我们的结果,ID Screen® ELISA 与 WB 具有高度一致性,并且比 BIO K302 ELISA 具有更高的精度和准确性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a6/6814985/8570e6a46c47/12917_2019_2117_Fig1_HTML.jpg

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