Department of Clinical Veterinary Medicine, Jilin University, Changchun, Jilin Province, People's Republic of China.
Int Immunopharmacol. 2013 Oct;17(2):478-82. doi: 10.1016/j.intimp.2013.07.010. Epub 2013 Aug 5.
Mastitis is a prevalent and economic disease around the world and defined as infection and inflammation of the mammary gland. Astragalin, a bioactive component isolated from persimmon or Rosa agrestis, has been reported to have anti-inflammatory properties. To investigate the potential therapeutic effect of astragalin in mastitis, a murine model of mastitis was induced by administration of LPS in mammary gland. Astragalin was applied 1h before and 12h after LPS treatment. The results showed that astragalin attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO) and the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in a dose-dependent manner. Additionally, Western blotting results showed that astragalin efficiently blunt decreased nuclear factor-kappaB (NF-κB) activation by inhibiting the degradation and phosphorylation of IκBα and the nuclear translocation of p65. These results suggested that astragalin exerts anti-inflammatory properties in LPS-mediated mastitis, possibly through inhibiting inhibition of the NF-κB signaling pathway, which mediates the expression of pro-inflammatory cytokines. Astragalin may be a potential therapeutic agent against mastitis.
乳腺炎是一种在世界各地普遍存在且具有经济影响的疾病，被定义为乳腺的感染和炎症。山奈酚是一种从柿子或野蔷薇中分离出来的生物活性成分，据报道具有抗炎特性。为了研究山奈酚在乳腺炎中的潜在治疗作用，通过在乳腺中给予 LPS 诱导乳腺炎的小鼠模型。在 LPS 处理前 1 小时和后 12 小时应用山奈酚。结果表明，山奈酚以剂量依赖性方式减轻了炎症细胞的浸润、髓过氧化物酶（MPO）的活性以及肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的表达。此外，Western blotting 结果表明，山奈酚通过抑制 IκBα 的降解和磷酸化以及 p65 的核易位，有效地阻止了核因子-κB（NF-κB）的激活。这些结果表明，山奈酚在 LPS 介导的乳腺炎中发挥抗炎作用，可能通过抑制 NF-κB 信号通路来介导促炎细胞因子的表达。山奈酚可能是一种治疗乳腺炎的潜在治疗剂。
