Zhang Shaobin, Chen Hong, Hua Juan, Luo Shihong
Engineering Research Center of Protection and Utilization of Plant Resources, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, China.
Front Chem. 2024 Dec 2;12:1506792. doi: 10.3389/fchem.2024.1506792. eCollection 2024.
The Chinese medicinal plant Turcz. is the only plant used in the manufacture of Bairui Granules. However, to date, there has been very little research into the cytotoxic activity of active substances derived from Bairui Granules. Using chemical separation and spectroscopic methods, phenolic compounds 1-5 were identified as methyl--hydroxycinnamate, vanillin, kaempferol, isorhamnetin-3--glucoside, and astragalin, respectively. UPLC-MS/MS analyses revealed that compounds 1-5 were present at concentrations of 0.006 ± 0.002, 1.63 ± 0.87, 3.65 ± 0.83, 26.97 ± 11.41, and 27.67 ± 2.91 g/g, respectively in Bairui Granules. Compounds 1, 2, and 4 were detected here for the first time in Bairui Granules. Using co-culture experiments, isorhamnetin-3--glucoside (4) was found to be beneficial to the proliferation Chinese hamster ovary (CHO) cells (6.46% ± 0.86% to 38.45% ± 9.04%), natural killer cells from human umbilical cord blood (UCB NK cells) (25.68% ± 0.02% to 70.81% ± 0.26%), and mesenchymal stem cells from human umbilical cord blood (UCB MSC cells) (1.66% ± 0.05% to 27.64% ± 0.51%) when the concentration was similar to that found in Bairui granules. Moreover, vanillin (2) was conducive to UCB NK cells proliferation (28.21% ± 0.44%) at a concentration of 64 g/mL, while maintaining cell viability. UCB NK cell proliferation was promoted at rates of 41.03% ± 0.48% to 67.22% ± 0.68% when astragalin (5) was present at low concentrations (8 and 16 g/mL). Methyl--hydroxycinnamate (1) and vanillin (2) at different concentrations both had an inhibitory effect on the proliferation of natural killer cells from human peripheral blood (PB NK cells), but the inhibitory concentration ranges of these compounds were not equivalent to the concentration ranges of the compounds in Bairui Granules. These results provide a foundation for the safe use of preparations.
中药植物Turcz.是制造百蕊颗粒唯一使用的植物。然而，迄今为止，对百蕊颗粒活性物质的细胞毒性活性研究很少。采用化学分离和光谱方法，分别鉴定出酚类化合物1 - 5为甲基 - 羟基肉桂酸酯、香草醛、山柰酚、异鼠李素 - 3 - 葡萄糖苷和紫云英苷。超高效液相色谱 - 串联质谱分析表明，化合物1 - 5在百蕊颗粒中的浓度分别为0.006±0.002、1.63±0.87、3.65±0.83、26.97±11.41和27.67±2.91μg/g。化合物1、2和4首次在百蕊颗粒中被检测到。通过共培养实验发现，当浓度与百蕊颗粒中浓度相似时，异鼠李素 - 3 - 葡萄糖苷（4）对中国仓鼠卵巢（CHO）细胞（从6.46%±0.86%至38.45%±9.04%）、人脐带血自然杀伤细胞（UCB NK细胞）（从25.68%±0.02%至70.81%±0.26%）和人脐带血间充质干细胞（UCB MSC细胞）（从1.66%±0.05%至27.64%±0.51%）的增殖有益。此外，香草醛（2）在浓度为64μg/mL时有利于UCB NK细胞增殖（28.2l%±0.44%），同时维持细胞活力。当紫云英苷（5）处于低浓度（8和16μg/mL）时，UCB NK细胞增殖促进率为41.03%±0.48%至67.22%±0.68%。不同浓度的甲基 - 羟基肉桂酸酯（1）和香草醛（2）均对人外周血自然杀伤细胞（PB NK细胞）的增殖有抑制作用，但这些化合物的抑制浓度范围与百蕊颗粒中化合物的浓度范围不等同。这些结果为该制剂的安全使用提供了依据。
