Lötsch Jörn, Schaeffeler Elke, Mittelbronn Michel, Winter Stefan, Gudziol Volker, Schwarzacher Stephan W, Hummel Thomas, Doehring Alexandra, Schwab Matthias, Ultsch Alfred
Institute of Clinical Pharmacology, Goethe-University, Theodor Stern Kai 7, 60590, Frankfurt am Main, Germany,
Brain Struct Funct. 2014 Nov;219(6):1991-2000. doi: 10.1007/s00429-013-0618-3. Epub 2013 Aug 9.
The human olfactory bulb displays high morphologic dynamics changing its volume with olfactory function, which has been explained by active neurogenetic processes. Discussion continues whether the human olfactory bulb hosts a continuous turnover of neurons. We analyzed the transcriptome via RNA quantification of adult human olfactory bulbs and intersected the set of expressed transcriptomic genes with independently available proteomic expression data. To obtain a functional genomic perspective, this intersection was analyzed for higher-level organization of gene products into biological pathways established in the gene ontology database. We report that a fifth of genes expressed in adult human olfactory bulbs serve functions of nervous system or neuron development, half of them functionally converging to axonogenesis but no other non-neurogenetic biological processes. Other genes were expectedly involved in signal transmission and response to chemical stimuli. This provides a novel, functional genomics perspective supporting the existence of neurogenesis in the adult human olfactory bulb.
人类嗅球表现出高度的形态动力学变化，其体积随嗅觉功能而改变，这已通过活跃的神经遗传过程得到解释。关于人类嗅球是否存在神经元的持续更新仍在讨论中。我们通过对成年人类嗅球进行RNA定量分析了转录组，并将表达的转录组基因集与独立可得的蛋白质组表达数据进行了交叉分析。为了获得功能基因组学视角，对该交叉分析结果进行了分析，以了解基因产物在基因本体数据库中建立的生物途径中的高级组织情况。我们报告称，成年人类嗅球中表达的基因有五分之一发挥着神经系统或神经元发育的功能，其中一半在功能上汇聚于轴突形成，但没有其他非神经遗传的生物过程。其他基因如预期的那样参与信号传递和对化学刺激的反应。这提供了一个新的功能基因组学视角，支持成年人类嗅球中存在神经发生。
