Shlush Liran I, Selig Sara
Campbell Family Cancer Research Institute, Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Canada.
Methods Mol Biol. 2013;1048:11-8. doi: 10.1007/978-1-62703-556-9_2.
Cellular senescence plays important roles in the aging process of complex organisms, in response to stress and in tumor suppression. Several markers can be used to identify senescent cells, of which the most widely used is the senescence-associated β-galactosidase (SABG) activity. Here we describe a procedure for digital image analysis of cells stained by the SABG staining technique at pH 6 or at pH 4. This analysis is highly reproducible and sensitive to subtle differences in staining intensities resulting from diverse cellular senescence pathways in culture.
细胞衰老在复杂生物体的衰老过程、应激反应和肿瘤抑制中发挥着重要作用。有几种标志物可用于识别衰老细胞，其中使用最广泛的是衰老相关β-半乳糖苷酶（SABG）活性。在此，我们描述了一种对用SABG染色技术在pH 6或pH 4条件下染色的细胞进行数字图像分析的方法。这种分析具有高度可重复性，并且对培养中不同细胞衰老途径导致的染色强度细微差异敏感。
