使用腺相关病毒载体的线粒体靶向抗衰老基因疗法。
Mitochondria-targeted antiaging gene therapy with adeno-associated viral vectors.
作者信息
Li Dejia, Duan Dongsheng
机构信息
Department of Occupational Environmental Health, School of Public Health, Wuhan University, Wuhan, People's Republic of China.
出版信息
Methods Mol Biol. 2013;1048:161-80. doi: 10.1007/978-1-62703-556-9_13.
Abstract
Transgenic expression of catalase in mitochondria using a transgenic strategy extends life span and prevents aging-related pathology in mice. However, transgenic overexpression is not suitable for a clinical application. Adeno-associated virus (AAV) is the most promising gene delivery vehicle. Here we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.
摘要
利用转基因策略使过氧化氢酶在线粒体中进行转基因表达可延长小鼠寿命并预防与衰老相关的病理变化。然而，转基因过表达并不适用于临床应用。腺相关病毒（AAV）是最具前景的基因传递载体。在此，我们概述了生成表达线粒体靶向过氧化氢酶基因（AV.RSV.MCAT）的AAV载体的策略。我们还描述了评估AV.RSV.MCAT对小鼠肌肉收缩力和跑步能力的生理影响的方法。
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