Kimelman Nadav Bleich, Kallai Ilan, Sheyn Dmitriy, Tawackoli Wafa, Gazit Zulma, Pelled Gadi, Gazit Dan
Skeletal Biotech Laboratory, The Hebrew University-Hadassah Faculty of Dental Medicine, Ein Kerem, Jerusalem, Israel.
Methods Mol Biol. 2013;1048:181-93. doi: 10.1007/978-1-62703-556-9_14.
Real-time bioluminescence functional imaging holds great promise for regenerative medicine because it improves the researcher's ability to analyze and understand the healing process. Using transgenic mice coupled with gene-modified cells, one can employ this method to monitor host and graft activity in various models of tissue regeneration. We implemented real-time bioluminescence functional imaging to analyze bone formation by following a unique protocol in which the luciferase reporter gene, driven by an osteocalcin promoter, is used to visualize host and graft activity during bone formation. Real-time bioluminescence functional imaging can be used to assess the "host reaction" in transgenic mice models; it can also be used to assess "graft activity" in other animals in which genetically labeled stem cells have been implanted or direct gene delivery has been applied. The suggested imaging protocol requires 25 min per sample. However, special attention must be given to the layout of the experimental design, which determines the specific activity that will be analyzed.
实时生物发光功能成像在再生医学领域极具前景，因为它提高了研究人员分析和理解愈合过程的能力。通过将转基因小鼠与基因修饰细胞相结合，人们可以运用这种方法在各种组织再生模型中监测宿主和移植物的活性。我们采用实时生物发光功能成像，按照一种独特的方案来分析骨形成过程，在该方案中，由骨钙素启动子驱动的荧光素酶报告基因用于在骨形成过程中可视化宿主和移植物的活性。实时生物发光功能成像可用于评估转基因小鼠模型中的“宿主反应”；它还可用于评估其他动物中的“移植物活性”，这些动物已植入基因标记的干细胞或已应用直接基因递送。建议的成像方案每个样本需要25分钟。然而，必须特别注意实验设计的布局，它决定了将要分析的具体活性。
