人脐血单个核细胞对匹罗卡品诱导癫痫模型的抗癫痫和神经保护作用。
Antiepileptic and neuroprotective effects of human umbilical cord blood mononuclear cells in a pilocarpine-induced epilepsy model.
机构信息
Centro de Biotecnologia e Terapia Celular, Hospital São Rafael, Av. S Rafael, Salvador, BA, 2152-41253-190, Brazil.
出版信息
Cytotechnology. 2014 Mar;66(2):193-9. doi: 10.1007/s10616-013-9557-3. Epub 2013 Aug 9.
Abstract
Status epilepticus (SE) is a condition of persistent seizure that leads to brain damage and, frequently, to the establishment of chronic epilepsy. Cord blood is an important source of adult stem cells for the treatment of neurological disorders. The present study aimed to evaluate the effects of human umbilical cord blood mononuclear cells (HUCBC) transplanted into rats after induction of SE by the administration of lithium and pilocarpine chloride. Transplantation of HUCBC into epileptic rats protected against neuronal loss in the hippocampal subfields CA1, CA3 and in the hilus of the dentate gyrus, up to 300 days after SE induction. Moreover, transplanted rats had reduced frequency and duration of spontaneous recurrent seizures (SRS) 15, 120 and 300 days after the SE. Our study shows that HUCBC provide prominent antiepileptic and neuroprotective effects in the experimental model of epilepsy and reinforces that early interventions can protect the brain against the establishment of epilepsy.
摘要
癫痫持续状态（SE）是一种持续癫痫发作的状态，可导致脑损伤，并经常导致慢性癫痫的发生。脐血是治疗神经紊乱的成人干细胞的重要来源。本研究旨在评估在 SE 诱导后通过给予氯化锂和匹罗卡品，将人脐血单核细胞（HUCBC）移植到大鼠体内的效果。将 HUCBC 移植到癫痫大鼠体内，可以防止 SE 诱导后 300 天内海马 CA1、CA3 区和齿状回门区的神经元丢失。此外，移植后的大鼠在 SE 后 15、120 和 300 天，自发性反复发作性癫痫（SRS）的频率和持续时间降低。我们的研究表明，HUCBC 在癫痫实验模型中提供了显著的抗癫痫和神经保护作用，并且强化了早期干预可以保护大脑免受癫痫的发生。
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