Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, Berlin, D-14195, Germany.
Macromol Rapid Commun. 2013 Sep;34(17):1401-7. doi: 10.1002/marc.201300353. Epub 2013 Aug 9.
Supramolecular microgel capsules based on polyethylene glycol (PEG) are a promising class of soft particulate scaffolds with tailored properties. An approach to fabricate such particles with exquisite control by droplet-based microfluidics is presented. Linear PEG precursor polymers that carry bipyridine moieties on both chain termini are gelled by complexation to iron(II) ions. To investigate the biocompatibility of the microgels, living mammalian cells are encapsulated within them. The microgel elasticity is controlled by using PEG precursors of different molecular weights at different concentrations and the influence of these parameters on the cell viabilities, which can be optimized to exceed 90% is studied. Reversion of the supramolecular polymer cross-linking allows the microcapsules to be degraded at mild conditions with no effect on the viability of the encapsulated and released cells.
基于聚乙二醇 (PEG) 的超分子微凝胶胶囊是一类很有前途的软颗粒支架，具有可定制的性质。本文提出了一种通过基于液滴的微流控技术来制备具有精密控制的此类颗粒的方法。携带双吡啶基团的线性 PEG 前体聚合物通过与铁 (II) 离子络合而凝胶化。为了研究微凝胶的生物相容性，将活的哺乳动物细胞封装在其中。通过使用不同分子量的 PEG 前体在不同浓度下进行控制，可以控制微凝胶的弹性，研究这些参数对细胞活力的影响，细胞活力可以优化至超过 90%。超分子聚合物交联的反转允许在温和条件下降解微胶囊，而对封装和释放细胞的活力没有影响。
