Freie Universität Berlin, Institute for Chemistry and Biochemistry, Takustrasse 3, 14195 Berlin (Germany).
Angew Chem Int Ed Engl. 2013 Dec 16;52(51):13538-43. doi: 10.1002/anie.201308005. Epub 2013 Nov 29.
pH-Cleavable cell-laden microgels with excellent long-term viabilities were fabricated by combining bioorthogonal strain-promoted azide-alkyne cycloaddition (SPAAC) and droplet-based microfluidics. Poly(ethylene glycol)dicyclooctyne and dendritic poly(glycerol azide) served as bioinert hydrogel precursors. Azide conjugation was performed using different substituted acid-labile benzacetal linkers that allowed precise control of the microgel degradation kinetics in the interesting pH range between 4.5 and 7.4. By this means, a pH-controlled release of the encapsulated cells was achieved upon demand with no effect on cell viability and spreading. As a result, the microgel particles can be used for temporary cell encapsulation, allowing the cells to be studied and manipulated during the encapsulation and then be isolated and harvested by decomposition of the microgel scaffolds.
通过结合生物正交应变促进叠氮-炔环加成（SPAAC）和基于液滴的微流控技术，制备了具有优异长期存活率的 pH 可裂解载细胞微凝胶。聚（乙二醇）二环辛炔和树枝状聚（甘油叠氮化物）用作生物惰性水凝胶前体。通过使用不同取代的酸不稳定苯乙缩醛连接物进行叠氮化物缀合，从而可以在有趣的 pH 范围 4.5 到 7.4 之间精确控制微凝胶的降解动力学。通过这种方式，可以按需实现封装细胞的 pH 控制释放，而对细胞活力和扩散没有影响。结果，微凝胶颗粒可用于临时细胞封装，允许在封装过程中对细胞进行研究和操作，然后通过微凝胶支架的分解将其分离和收获。
