一种微流控被动泵送库尔特计数器。
A Microfluidic Passive Pumping Coulter Counter.
作者信息
McPherson Amy L, Walker Glenn M
机构信息
Department of Biomedical Engineering, North Carolina State University, Raleigh & University of North Carolina at Chapel Hill, NC, Tel.: 919-513-8253
出版信息
Microfluid Nanofluidics. 2010 Oct 1;9(4-5):897-904. doi: 10.1007/s10404-010-0609-0.
A microfluidic device using on-chip passive pumping was characterized for use as a particle counter. Flow occurred due to a Young-Laplace pressure gradient between two 1.2 mm diameter inlets and a 4 mm diameter reservoir when 0.5 L fluid droplets were applied to the inlets using a micropipette. Polystyrene particles (10m diameter) were enumerated using the resistive pulse technique. Particle counts using passive pumping were within 13% of counts from a device using syringe pumping. All pumping methods produced particle counts that were within 16% of those obtained with a hemocytometer. The effect of intermediate wash steps on particle counts within the passive pumping device was determined. Zero, one, or two wash droplets were loaded after the first of two sample droplets. No statistical difference was detected in the mean particle counts among the loading patterns ( > 0.05). Hydrodynamic focusing using passive pumping was also demonstrated.
一种采用芯片上被动泵送的微流控装置被表征为用作粒子计数器。当使用微量移液器将0.5微升液滴施加到两个直径为1.2毫米的入口时，由于两个1.2毫米直径入口与一个4毫米直径储液器之间的杨 - 拉普拉斯压力梯度而产生流动。使用电阻脉冲技术对直径为10微米的聚苯乙烯颗粒进行计数。使用被动泵送的颗粒计数与使用注射器泵送的装置的计数相差在13%以内。所有泵送方法产生的颗粒计数与用血细胞计数器获得的计数相差在16%以内。确定了中间洗涤步骤对被动泵送装置内颗粒计数的影响。在两个样品液滴中的第一个之后加载零个、一个或两个洗涤液滴。在加载模式之间的平均颗粒计数中未检测到统计学差异（P>0.05）。还展示了使用被动泵送的流体动力聚焦。
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