[使用AutoMate Express法医DNA提取系统从骨骼和牙齿中提取DNA]
[DNA extraction from bones and teeth using AutoMate Express forensic DNA extraction system].
作者信息
Gao Lin-Lin, Xu Nian-Lai, Xie Wei, Ding Shao-Cheng, Wang Dong-Jing, Ma Li-Qin, Li You-Ying
机构信息
Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310004, China.
出版信息
Fa Yi Xue Za Zhi. 2013 Apr;29(2):127-9.
OBJECTIVE
To explore a new method in order to extract DNA from bones and teeth automatically.
METHODS
Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system.
RESULTS
DNA extraction from bones and teeth were completed in 3 hours using the AutoMate Express forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method.
CONCLUSION
AutoMate Express forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.
目的
探索一种从骨骼和牙齿中自动提取DNA的新方法。
方法
分别采用冷冻研磨法和手工法获取33份骨骼样本和15份牙齿样本。使用AutoMate Express法医DNA提取系统从研磨后的样本中提取DNA并进行定量。
结果
使用AutoMate Express法医DNA提取系统在3小时内完成了从骨骼和牙齿中提取DNA的工作。两种方法提取的骨骼DNA浓度无统计学差异。冷冻研磨法对骨骼和牙齿均获得了良好的STR分型，且牙齿的DNA浓度高于手工法。
结论
AutoMate Express法医DNA提取系统是一种从骨骼和牙齿中提取DNA的新方法，可应用于法医实践。
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