Liu Jason Y, Zhong Chang, Holt Allison, Lagace Robert, Harrold Michael, Dixon Alan B, Brevnov Maxim G, Shewale Jaiprakash G, Hennessy Lori K
Life Technologies Corporation, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
J Forensic Sci. 2012 Jul;57(4):1022-30. doi: 10.1111/j.1556-4029.2012.02084.x. Epub 2012 Mar 5.
The AutoMate Express™ Forensic DNA Extraction System was developed for automatic isolation of DNA from a variety of forensic biological samples. The performance of the system was investigated using a wide range of biological samples. Depending on the sample type, either PrepFiler™ lysis buffer or PrepFiler BTA™ lysis buffer was used to lyse the samples. After lysis and removal of the substrate using LySep™ column, the lysate in the sample tubes were loaded onto AutoMate Express™ instrument and DNA was extracted using one of the two instrument extraction protocols. Our study showed that DNA was recovered from as little as 0.025 μL of blood. DNA extracted from casework-type samples was free of detectable PCR inhibitors and the short tandem repeat profiles were complete, conclusive, and devoid of any PCR artifacts. The system also showed consistent performance from day-to-day operation.
AutoMate Express™法医DNA提取系统专为从各种法医生物样本中自动分离DNA而开发。使用多种生物样本对该系统的性能进行了研究。根据样本类型，使用PrepFiler™裂解缓冲液或PrepFiler BTA™裂解缓冲液裂解样本。使用LySep™柱裂解并去除底物后，将样品管中的裂解物加载到AutoMate Express™仪器上，并使用两种仪器提取方案之一提取DNA。我们的研究表明，从低至0.025μL的血液中即可回收DNA。从实际案件样本中提取的DNA不含可检测到的PCR抑制剂，短串联重复序列图谱完整、确凿，且无任何PCR假象。该系统在日常操作中也表现出一致的性能。
