荧光蛋白在显微镜学中的应用。

Fluorescent protein applications in microscopy.

作者信息

Johnson Whitney L, Straight Aaron F

机构信息

Department of Biochemistry, Stanford Medical School, Stanford University, Stanford, California, USA.

出版信息

Methods Cell Biol. 2013;114:99-123. doi: 10.1016/B978-0-12-407761-4.00005-1.

DOI:10.1016/B978-0-12-407761-4.00005-1

PMID:23931504

Abstract

The use of fluorescent proteins (FPs) in modern cell biology and microscopy has had an extraordinary impact on our ability to investigate dynamic processes in living cells. FPs are unique in that fluorescence is encoded solely by the primary amino acid sequence of the FP and does not require enzymatic modification or cofactors. This genetically encoded fluorescence enables the expression of FPs in diverse cells and organisms and the detection of that fluorescence in living systems. This chapter focuses on microscopy-based applications of FP detection to monitor protein localization, dynamics, interaction, and the cellular environment.

摘要

荧光蛋白（FPs）在现代细胞生物学和显微镜技术中的应用，对我们研究活细胞动态过程的能力产生了非凡的影响。荧光蛋白的独特之处在于，其荧光仅由荧光蛋白的一级氨基酸序列编码，无需酶促修饰或辅助因子。这种基因编码的荧光使得荧光蛋白能够在多种细胞和生物体中表达，并能在活体系统中检测到这种荧光。本章重点介绍基于显微镜技术的荧光蛋白检测应用，以监测蛋白质的定位、动态变化、相互作用以及细胞环境。