Boaro Andreia, Ageitos Lucía, Torres Marcelo, Bartoloni Fernando Heering, de la Fuente-Nunez Cesar
Machine Biology Group, Departments of Psychiatry and Microbiology, Institute for Biomedical Informatics, Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, and Department of Bioengineering, University of Pennsylvania, 3610 Hamilton Walk, Philadelphia, PA 19104, USA.
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Avenida dos Estados, 5001, Santo André, São Paulo 09210-580, Brazil.
Cell Rep Phys Sci. 2020 Dec 23;1(12). doi: 10.1016/j.xcrp.2020.100257. Epub 2020 Nov 25.
Peptides are versatile biopolymers composed of 2-100 amino acid residues that present a wide range of biological functions and constitute potential therapies for numerous diseases, partly due to their ability to penetrate cell membranes. However, their mechanisms of action have not been fully elucidated due to the lack of appropriate tools. Existing light-emitting probes are limited by their cytotoxicity and large size, which can alter peptide structure and function. Here, we describe the available fluorescent, bioluminescent, and chemiluminescent probes for labeling peptides, with a focus on minimalistic options.
肽是由2至100个氨基酸残基组成的多功能生物聚合物，具有广泛的生物学功能，并且构成了许多疾病的潜在治疗方法，部分原因是它们具有穿透细胞膜的能力。然而，由于缺乏合适的工具，它们的作用机制尚未完全阐明。现有的发光探针受到其细胞毒性和大尺寸的限制，这可能会改变肽的结构和功能。在这里，我们描述了用于标记肽的可用荧光、生物发光和化学发光探针，重点是简约型选项。
