Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
Vet Microbiol. 2013 Oct 25;166(3-4):535-42. doi: 10.1016/j.vetmic.2013.06.023. Epub 2013 Jul 9.
MucR is a transcriptional regulator in many bacterial pathogens and is required for virulence in mice and macrophages, resistance to stress responses, and modification of the cell envelope in Brucella spp. To determine why the mucR deleted mutant is attenuated in vivo and in vitro, we performed RNA-seq analysis using Brucella melitensis RNA obtained from B. melitensis 16M and 16MΔmucR grown under the same conditions. We found 442 differentially expressed genes; 310 were over expressed, and 132 were less expressed in 16MΔmucR. Many genes identified are involved in metabolism, cell wall/envelope biogenesis, replication, and translation. Notably, genes involved in type IV secretion system and quorum sensing system were down-regulated in 16MΔmucR. In addition, genes involved in tolerance to acid and iron-limitation were also affected and experimentally verified in this study. The effects of MucR on Brucella survival and persistence in mice and macrophages were related to type IV secretion system, quorum sensing system, and stress tolerance, which also provide added insight to the MucR regulon.
MucR 是许多细菌病原体中的转录调节剂，对于鼠和巨噬细胞中的毒力、应激反应的抗性以及布鲁氏菌属细胞包膜的修饰都是必需的。为了确定为什么 mucR 缺失突变体在体内和体外都衰减，我们使用 Brucella melitensis RNA 进行了 RNA-seq 分析，这些 RNA 是从在相同条件下生长的 B. melitensis 16M 和 16MΔmucR 中获得的。我们发现了 442 个差异表达基因；在 16MΔmucR 中，有 310 个基因过表达，132 个基因表达量降低。鉴定出的许多基因参与代谢、细胞壁/包膜生物发生、复制和翻译。值得注意的是，16MΔmucR 中下调了与 IV 型分泌系统和群体感应系统相关的基因。此外，本研究还通过实验验证了与酸和铁限制耐受相关的基因也受到了影响。MucR 对布鲁氏菌在小鼠和巨噬细胞中存活和持续存在的影响与 IV 型分泌系统、群体感应系统和应激耐受有关，这也为 MucR 调控子提供了更多的见解。
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